HOXA-10、HOXA-11、Esr2、Pgr在控制性超排卵小鼠围着床期子宫内膜表达的研究  

Study of HOXA-10、HOXA-11、Esr2、Pgr expression in endometrium during the peri-implatation period after controlled ovarian hyperstimulation on mice

作　　者：付正英[1] 卢建荣[1,2] 苏晓华[1,2] 

机构地区：[1]武警后勤学院附属医院,天津300162 [2]天津中医药大学,天津300193

出　　处：《现代妇产科进展》2012年第12期945-948,共4页Progress in Obstetrics and Gynecology

基　　金：武警后勤学院课题(No:WYM201116)

摘　　要：目的:检测同源框基因HOXA-10、HOXA-11以及雌激素受体Esr2、孕激素受体Pgr在控制性超排卵(COH)小鼠围着床期子宫内膜中的表达,探讨COH对子宫内膜容受性的影响。方法:将30只SPF级雌性昆明小鼠随机分为模型组(GnRH-a+HMG+HCG)和对照组(生理盐水),每组15只,造模成功后,计算子宫脏器指数,光学显微镜观察子宫内膜组织学变化;应用RT-PCR和Real-time PCR方法检测小鼠子宫内膜中HOXA-10、HOXA-11、Esr2、Pgr mRNA的表达。结果:模型组围着床期小鼠子宫脏器指数较对照组显著降低(P<0.05),子宫内膜发育延迟,子宫内膜成熟度及厚度降低;模型组小鼠HOXA-10、HOXA-11、Pgr mRNA表达较对照组显著下降(P<0.05),而Esr2 mRNA表达显著升高(P<0.05)。结论:COH引起HOXA-10、HOXA-11、Pgr mRNA表达下降,Esr2mRNA表达升高,致使围着床期内膜准备不足,子宫内膜容受性下降。Objective：To study the expressions of HOXA-10,HOXA-11 ,Esr2 and Pgr in endometrium during the peri-implatation period after controlled ovarian hyperstimulation on mice. To explore the influence of COH to endometrial receptivity. Methods：Thirty female mice were randomized into the treatment group （GnRH-a ＋ HMG ＋ HCG） and the control group （0.9% NS）, 15 cases in every group. After successful modeling, calculate the uterus index,ob- served the endometrial histology with Optical Microscope. Detection of HOXA-10 ,HOXA-11 Esr2, Pgr mRNA expression in endometrial with Polymerase Chain Reaction and Real-time Poly- merase Chain Reaction. Results：The uterus index were decreased significantly and development delay in endometrium,maturity and thickness decreased, HOXA-10、HOXA-11 and Pgr mRNA expressions were decreased,but Esr2 mRNA expression was higher,in treatment group of COH mice（P〈0.05 ）. There were significantly differences compared with the control group （P〈 0.05 ）. Conclusion ： COH lead to HOXA-10、HOXA-11、Pgr mRNA expressions decreased and Esr2 mRNA expression higher in endometrial, endometrial insufficient preparation and endome- trial receptivity decreased.

关 键 词：胚胎植入 控制性超排卵(COH) 子宫内膜容受性 小鼠 HOXA-10 HOXA-11 Esr2 Pgr 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]