小鼠磨牙牙胚发育过程中硫酸软骨素硫酸化模式的时空变化  

Temporal-spatial Changes of the Sulfated Patterns of Chondroitin Sulfate during the Development of the Embryonic Mouse Molars

作　　者：蒋备战[1] 王佐林[2] 

机构地区：[1]同济大学口腔医学院牙体牙髓病学教研室,上海200072 [2]同济大学口腔医学院口腔颌面外科教研室

出　　处：《口腔医学研究》2012年第12期1214-1216,1220,共4页Journal of Oral Science Research

基　　金：上海市科学技术委员会医学引导项目(编号:114119a3400)

摘　　要：目的:检测硫酸软骨素在小鼠磨牙牙胚发育过程中的硫酸化模式的时空变化。方法:取胎龄分别为E11.5、E13.5、E15.0、E16.5和E18.5d的ICR胎鼠头部,常规组织学处理,制备5μm厚矢状或冠状切片,用免疫组织化学方法检测4-硫酸软骨素、6-硫酸软骨素在下颌第一磨牙牙胚组织中的时空表达。结果:从小鼠牙胚开始发生到帽状期,4-硫酸软骨素只在牙源性上皮外层细胞与基底膜强表达,在牙源性间充质组织中不表达,钟状早期其开始在牙源性间充质组织中表达。6-硫酸软骨素只在牙源性间充质细胞中表达,且其与4-硫酸软骨素呈互补表达模式。结论:硫酸软骨素在牙胚发生过程中其硫酸化模式呈现时间-空间的特异性改变,这种特异性改变可能与小鼠磨牙牙胚的形态发生密切相关。Objective：To study the temporal-spatial changes of the sulfated patterns of chondroitin sulfate in the embryonic first lower mouse molars.Methods：5μm serial paraffin sections of the first lower molars at different embryonic stages were made.The expression patterns of 4-chondroitin sulfate（C4S） and 6-chondroitin sulfate（C6S） were analyzed by immunohistochemistry.Results：C4S was restrictively expressed in the dental epithelium and the base membrane from the tooth initial stage to the cap stage.From the early bell stage,C4S was able to be detected in the dental mesenchyme cells which was adjacent to the inner enamel cells.C6S was never detected in the dental epithelium,and weakly expressed in the dental mesenchyme at the initial stage.With the development of the tooth germs.the expression of C6S in the dental mesenchyme increased gradually,after the onset of cusp formation,C6S was disappeared from the occlusive regions,while C4S was intense expressed.It seemed that the expression patterns of C4S and C6S in the dental papilla were mutually complementary.Conclusion：The expression patterns of C4S and C6S were temporal and spatial specific.This specific patterns might be involved in the tooth morphogenesis of the embryonic mouse molars.

关 键 词：硫酸软骨素 牙胚发育 小鼠 磨牙 免疫组化 

分 类 号：R780.2[医药卫生—口腔医学]