锌对体外培养小鼠腭胚突融合影响的初步研究  

Effect of Zn on Mouse Embryo Palate Fusion by Culture in Vitro

作　　者：陈尚[1] 牟禄瑞[1] 王芳[1] 蒋程程[1] 宋庆高[1] 

机构地区：[1]遵义医学院附属口腔医院口腔颌面外科,贵州遵义563000

出　　处：《口腔医学研究》2012年第12期1225-1227,1230,共4页Journal of Oral Science Research

摘　　要：目的:通过研究不同浓度锌对体外培养小鼠腭胚突腭融合的影响以及锌对全反式维甲酸诱导胎鼠腭裂的拮抗作用,探讨锌在腭发育过程中的作用,为预防或减少腭裂的发生提供理论及实验基础。方法:获取胚胎13dC57BL/6N胎鼠腭突进行体外培养,分为空白对照组、低锌组、中锌组、高锌组、全反式维甲酸组及全反式维甲酸+中锌组,培养基中分别含锌离子0μmol/L、50μmol/L、75μmol/L、95μmol/L、全反式维甲酸10μmol/L及全反式维甲酸10μmol/L+锌离子75μmol/L,分别培养48h后进行组织学观察其形态学变化并计数腭板融合数。结果:空白对照组、低锌组、中锌组、高锌组、全反式维甲酸组及全反式维甲酸+中锌组腭板融合率分别为27.8%、53.9%、85%、32%、0%和6.7%,各组之间进行两两比较发现,空白对照组、低锌组和高锌组之间无明显的差异,而中锌组腭板融合率与各组之间有明显差异(P<0.05);空白对照组、全反式维甲酸组及全反式维甲酸+中锌组之间无明显的差异。结论:体外培养条件下锌浓度达75μmol/L时能有效地促进腭突融合,但锌在预防全反式维甲酸诱导腭裂发生的过程中效果不佳。Objective：To explore the effect of Zn during palatogenesis by culturing mouse embryo palate in vitro under different consistence Zn and using Zn to rescue cleft palate induced all-trans retinoic acid and provide the theoretical and experimental basis for preventing and decrease cleft palate.Methods：We cultured C57BL/6N mouse embryo palate at embryo 13 and divided the mouse embryo palate into six groups：control（0μmol/L）,low Zn concentration（50μmol/L）,moderate Zn concentration（75μmol/L）,high Zn concentration（95μmol/L）,trans retinoic acid（10μmol/L） and all-trans retinoic acid（10μmol/L）＋moderate Zn（75μmol/L）.All sample were observed palatal morphology and recorded the number of palatal fusion after culturing 48 hours.Results：Rates of palatal fusion in control group,low Zn concentration group,moderate Zn concentration group,high Zn concentration group,all-trans retinoic acid group and all-trans retinoic acid ＋moderate Zn group were 27.8%,53.9%,85%,32%,0% and 6.7% respectively.The fusional rates were not significant different among control group,low Zn concentration group,high Zn concentration group and control group,all-trans retinoic acid group and all-trans retinoic acid ＋moderate Zn group.But there was significant difference between moderate Zn concentration and other group（P0.05）.Conclusion：75μmol/L Zn was the best concentration that can improve mouse embryo palate fusion under culturing in vitro.But Zn could not recuse cleft palate induced by all-trans retinoic acid.

关 键 词：微量元素锌 腭裂 全反式维甲酸 

分 类 号：R782.2[医药卫生—口腔医学]