人群膳食营养结构中维生素A的评价  

Evaluation of vitamin A in dietary and nutritional structure

作　　者：徐燕[1] 王冬梅[1] 

机构地区：[1]河南省安阳市疾病预防控制中心,河南安阳455000

出　　处：《中国卫生检验杂志》2012年第12期2839-2841,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立高效液相色谱法(high performance liquid chromatography,HPLC)测定等量复份膳食中维生素A含量的测定方法。方法:称取10 g样品于平底烧瓶中,加入淀粉酶,混匀,保持45℃30 min。经没食子酸乙醇-氢氧化钠皂化,石油醚提取,40℃通氮气情况下旋转蒸发近干,石油醚溶解、定容,再用氮气吹干,甲醇定容。HPLC法分析,保留时间定性,标准曲线法定量。分析柱为ODS柱(250 mm×46 mm,5μm),流动相:甲醇∶水(98∶2),检测波长325 nm。结果:维生素A在0.25μg/ml～110μg/ml之间线性良好(r>0.9994),平均回收率为97.3%,RSD为1.2%。结论:HPLC法测定等量复份膳食中维生素A的含量简便、快速,灵敏度高,准确性好。Objective：The method for determination of Vitamin A in equivalent duplicate diet by HPLC（High Performance Liquid Chromatography） was constructed.Methods： 10 g samples were weighed into florence flask and amylase was added.After well mixing and keeping 30 minute at 45℃,the samples were saponified by gallic acid ethanol-sodium hydroxide and extracted by petroleum ether,then rotary evaporated close to dry by nitrogen-blow at 40℃,dissolved and diluted with petroleum ether to volume,then evaporated to dry by nitrogen-blow again and diluted with methanol to volume.HPLC was employed for analysis,and retention time for qualitation and standard curve for quantitation.The analysis column of this method was ODS column（250 mm×46 mm,5 μm）,the mobile phase was methanol∶ H2O（98∶ 2） and detection wavelength was 325 nm.Results： The calibration plot was found to be linear in the range of 0.25 μg/ml^110 μg/ml for Vitamin A（r0.9994）.The average recovery was 97.3% and RSD was 1.2%.Conclusion： The method for determination of Vitamin A in equivalent duplicate diet by HPLC was simple,sensitive,rapid and accurate.

关 键 词：高效液相色谱法 等量复份膳食 维生素A 

分 类 号：R151.42[医药卫生—营养与食品卫生学]