ELISA法测定贝毒素污染的研究  

Detection of paralytic shellfish poisoning(PSP),diarrhetic shellfish poison(DSP),neurotoxic shellfish poison(NSP) by ELISA

作　　者：柳其芳[1] 

机构地区：[1]深圳市疾病预防控制中心理化检验科,广东深圳518055

出　　处：《中国热带医学》2012年第11期1398-1399,共2页China Tropical Medicine

摘　　要：目的探讨以ELISA检测麻痹性贝毒素(PSP)、腹泻性贝毒素(DSP)、神经性贝毒素(NSP)的可行性,并对深圳市污染状况调查。方法采用ELISA检测PSP、DSP、NSP,对抽样的深圳市贝类样品进行检测。结果 PSP浓度为0.02～0.32μg/kg之间的标准曲线R=0.996,变异系数(CV,%)为2.4～8.3,回收率为88.7%～105.0%;DSP浓度为0.20～5.00μg/kg之间的标准曲线R=0.995,变异系数(CV,%)为1.5～8.0,回收率为89.6%～116.0%;NSP浓度为0.10～2.50μg/kg之间的标准曲线R=0.993,变异系数(CV,%)为2.2～9.5,回收率为88.7%～111.6%。结论 ELISA法检测三种贝毒均具有灵敏度高、干扰少、测定步骤简便、快速、操作安全、结果准确可靠的优点,适合作为快速筛选方法。Objective To probe into the feasibility of detecting paralytic shellfish poisoning（PSP）,diarrhetic shellfish poison（DSP）,neurotoxic shellfish poison（NSP） by ELISA.Methods The competitive enzyme-linked immunosorbent assay（ELISA）was adopted for detection of PSP,DSP and NSP,The results were analyzed.Results For detection of PSP,the standard curve with concentration of 0.02~0.32μg/kg showed an R value of 0.996.Coefficient of variation（CV）was 2.4%-8.3% and recovery rate was 88.7%-105.0%;For detection of DSP,the standard curve with concentration of 0.20-5.00μg/kg showed an R value of 0.995 % and the CV was 1.5%-8.0 %,the recovery rate（%） was 89.6%-116.0%;For detection of NSP,the standard curve with concentration of 0.10-2.50μg/kg showed an R value of 0.993.CV was 2.2%-9.5% and recovery rate was 88.7%-111.6%.Conclusion ELISA detection was characterized by high sensitivity,low interference,simple and safe in operation,with rapid and reliable test results.in detection of PSP,DSP and NSP in fodder both at home and abroad.

关 键 词：麻痹性贝毒(PSP) 腹泻性贝毒(DSP) 神经性贝毒(NSP) 酶联免疫法 液相色谱-质谱联用仪 

分 类 号：R155.5[医药卫生—营养与食品卫生学]