Does Muller Cell Differentiation Occur Prior to the Emergence of Synapses in Embryonic Turtle Retina？  

作　　者：Yolanda Segovia Rosa Maria Perez Norberto Mauricio Grzywacz Joaquin De Juan 

机构地区：[1]Departamento de Biotecnologia, Universidad de Alicante, Alicante 03080, Spain [2]Departamento de Enfermeria, Universidad de Alicante, Alicante 03080, Spain [3]Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089-111, USA

出　　处：《Journal of Life Sciences》2012年第11期1200-1205,共6页生命科学（英文版）

摘　　要：MOiler cells are the main glial cells in the retina, and are related to plexiform layer activity. Recent studies have demonstrated that Muller cells are involved in the synaptic conservation, plasticity, development and metabolism of glutamate. During turtle retinal development, layers, cells and synapses appear at different times. The aim of this research is to study the emergence of Muller cells during embryonic development and their relationship with the synaptogenesis. The authors used retinas from Trachemys scripta elegans embryos at stages S14, 18, 20, 23, and 26. Some retinas were processed with immunocytochemistry in order to detect the presence of glutamine synthetase in Muller cells, which was used as a marker of these ceils. Other retinas from the same stages were processed for ultrastructural studies. Samples were observed in confocal and transmission electron microscopes, respectively. The present results show that glutamine synthetase expression in MOiler cells occurs at S18, before the emergence of the retinal layers and the early synapses.

关 键 词：SYNAPTOGENESIS retinal development glutamine synthetase ULTRASTRUCTURE immunochemistry. 

分 类 号：Q954.673[生物学—动物学] S966.5[农业科学—水产养殖]