p21活化蛋白激酶1对喉癌细胞运动侵袭能力的影响  

The effect of PAK1 on migration and invasion of laryngeal cancer cells

作　　者：何国庆[1] 管强[1] 张喜军[2] 张杰武[1] 姬宏飞[3] 

机构地区：[1]哈尔滨医科大学附属第三医院头颈外科,哈尔滨150081 [2]哈尔滨医科大学附属第四医院耳鼻咽喉科 [3]哈尔滨医科大学肿瘤防治研究所

出　　处：《实用肿瘤学杂志》2012年第6期501-505,共5页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究面上项目（11541221）

摘　　要：目的探讨PAKl（p21 activated kinase1）在喉癌中的表达及其对喉癌细胞运动侵袭能力的影响。方法首先获取22例喉癌和癌旁新鲜组织标本，采用Western blot方法检测PAKl的蛋白表达情况，再对63例喉癌和癌旁黏膜正常组织石蜡标本进行免疫组化研究，检测PAK1蛋白的表达，最后采用siRNA技术干扰FaDu喉癌细胞系的PAK1表达，同时进行细胞运动和侵袭能力的分析。结果Westernblot和免疫组化结果表明，PAK1在喉癌组织中的表达明显高于癌旁正常组织。喉癌细胞经PAK1 siRNA干扰后，运动和侵袭能力明显降低。结论PAK1的激活可能参与喉癌的发生进展，下调PAK1的活性可降低喉癌细胞的运动和侵袭能力。Objective To investigate the expression of PAK1 in laryngeal cancer and its effect on migra-tion and invasion of laryngeal cancer cells. Methods Firstly, 22 samples of fresh laryngeal cancer tissues and ad-jacently normal laryngeal mucosa were collected to detect the expression of PAK1 by Western blot. Secondly, 63 paraffin embedded laryngeal tissues were analyzed by immunohistochemistry to detect the expression of PAK1. Fi-nally, We observed cell migration and invasion by knockdown of PAK1 in FaDu cell line via siRNA. Results The results from western blot and immunohistochemistry demonstrated that the expression of PAK1 in laryngeal cancer tissues was higher than that of normal mucosa. The cell migration and invasion of laryngeal squamous cell carcinoma was reduced after PAK1 was knocked down through siRNA. Conclusion The activation of PAK1 may contribute to the development and progression of laryngeal squamous cell carcinoma. Inhibition of PAK1 could de-crease cell migration and invasion capability of laryngeal squamous cell carcinoma.

关 键 词：PAK1 鳞状细胞癌 喉 运动 侵袭 

分 类 号：R739.65[医药卫生—肿瘤]