应用噬菌体随机肽库筛选肺癌血清标志物的研究  

Screening of the phage - display random peptide specific to the sera of patients with lung cancer

作　　者：李文辉[1] 苏丽菊[2] 杨桐树[1] 杨威[3] 钟志[1] 马玉杰[3] 孟晓燕[3] 徐宁[1] 

机构地区：[1]哈尔滨医科大学附属第三医院检验科,哈尔滨150081 [2]哈尔滨市第一医院检验科 [3]黑龙江省电力医院检验科

出　　处：《实用肿瘤学杂志》2012年第6期511-514,共4页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究项目（11551155）;黑龙江省青年科学基金项目（QC2011C041）

摘　　要：目的利用噬菌体随机12肽库筛选肺癌患者血清中标志物，对探索肺癌的早期诊断、鉴别诊断、临床治疗特别是实施针对肺癌的早期干预有重要的理论意义和应用价值。方法分别选取正常人、良性肿瘤患者及肺癌患者血清各35例，先用肺癌患者、良性肿瘤患者、正常人混合血清作为筛选配基，对噬菌体随机12肽库进行减性淘筛，获得肺癌血清特异性结合的噬菌体克隆，并用患者混合血清进行Dot—ELISA实验鉴定获得的噬菌体克隆，进而分别用肺癌患者及正常人血清各12例进一步鉴定阳性噬菌体的混合克隆，确定阳性噬菌体克隆与个体血清之间的结合情况。结果经减性筛选后，特异结合的噬菌体得到富集。随机挑取的12个噬菌体克隆，经鉴定均能与肺癌患者血清特异性结合，阳性率100％。用肺癌病人血清和正常人血清各12例分别与阳性混合克隆反应，结果显示12例肺癌病人血清均呈阳性，而12例正常人血清均为阴性，敏感性和特异性均为100％。结论通过筛选肺癌患者血清学标志物，获得与肺癌患者血清特异结合的噬菌体多肽，其可能用于制备肺癌诊断试剂，对于开展肺癌的早期诊断及早期干预有重要的理论意义和应用价值。Objective In order to screening the phage - display random 12 peptide specific to the lung cancer and explore early diagnosis, differential diagnosis and clinical treatment for lung cancer. Methods We sdected the serum of lung cancer patient, benign tumor and normal tumor, which was 35 case respectirely, Using the phage random 12 peptide library screening（ the lung cancer specific phage clones）, we pooled these serum as a screening ligand. Then, the Dot-ELISA is used to identify the lung cancer specific phage clones reactive to sera of the lung cancer patients and normal controls individually. Results Total 12 of the phage - display random 12 peptide are obtained by phage peptide library screening and the Dot - ELISA identification. Mix of positive cloning respectively with the 12 cases of lung cancer patients and 12 cases of normal serum reaction and 12 cases of lung cancer patients serum all positive, normal serum all negative, sensitivity of 100% and specificity of 100%. Con- clusion Lung cancer - specific peptides were screened by phage random peptide library maybe used to diagnosis the lung cancer, and implementing early detection and early intervention has important theoretical significance and application value of lung cancer.

关 键 词：肺癌 噬菌体随机肽库 血清标志物 

分 类 号：R734.2[医药卫生—肿瘤]