UBE2W抑制TNFα诱导的NF-κB转录活性  

The Inhibition of TNFα Induced NF-κB Transcription Activity by UBE2W

作　　者：王少鑫[1] 巩伟丽[1] 陈媛[1] 靳宝锋[1] 

机构地区：[1]国家生物医学分析中心,北京100850

出　　处：《科学技术与工程》2012年第35期9457-9460,共4页Science Technology and Engineering

基　　金：国家自然科学基金项目(31071234)资助

摘　　要：UBE2W(ubiquitin-conjugating enzyme E2W)是一新的泛素结合酶,关于其功能研究甚少。采用双荧光报告系统和Western blot等方法,分别干涉和过表达UBE2W,分析UBE2W对TNF(刺激下NF-(B转录活性的影响。结果显示UBE2W能够显著抑制TNFα诱导的NF-κB的转录活性,提示该基因可能参与NF-κB相关功能的调控。UBE2W is a novel ubiquitin-conjugating enzyme （E2）, and its function has not been well defined. Dual-luciferase reporter assay system （pNF-KB-luc） and Western blot were used to evaluate the effects of UBE2W on the TNFα-induced NF-KB transcription activity when UBE2W was knocked down with siRNA or overex- pressed. The result indicates that UBE2W can significantly inhibit TNFα-induced NF-KB transcription activity and suggestes that UBE2W might participate in the regulation of NF-KB-associated functions.

关 键 词：UBE2W NF-ΚB TNFΑ 

分 类 号：Q559[生物学—生物化学]