miR-155反义寡核苷酸转染对人大肠癌Lovo细胞侵袭的影响  

Effects of miRNA-155 on invasion of human corectal cancer cell

作　　者：毛镇伟[1] 朱灵[1] 范钰[1] 

机构地区：[1]江苏大学附属人民医院肿瘤研究所,镇江212002

出　　处：《中国实用医药》2012年第34期43-44,共2页China Practical Medicine

基　　金：江苏省自然科学基金(项目编号:BK2009205);镇江市科技计划项目(项目编号:SH2009014)

摘　　要：目的探讨miR-155对人大肠癌细胞侵袭能力的影响。方法大肠癌Lovo细胞分为3组:脂质体介导反义miR-155(AS-miR-155)组、无义寡脱氧核苷酸(ODN)组和对照组。测定荧光素酶活性验证3组细胞中miR-155的表达,采用Matrigel基质生长试验检测细胞生长情况,以Transwe Ⅱ方法检测细胞的侵袭力,结果与对照组和无义ODN组比较,转染AS-miR-155组Lovo细胞miR-2l表达水平降低;Matrigel基质生长试验显示,转染AS-miR-155组Lovo细胞体外培养克隆平均直径较小,Transwe Ⅱ细胞侵袭试验显示转染AS-miR-155组穿膜细胞数较少。结论 miR-2l高表达可促进Lovo大肠癌细胞侵袭生长,提示miR-155可以作为基因治疗大肠癌的候选靶点。Objective To study the effects of miR-155 on invasion of human colon cells.Methods After human colon cancer Lovo cell were transfected by miR-155 antisense miRNA-155（AS-miR-155）.The miR-155 knocking down effects was examined by luciferase reporter assay.Matrigel cell growth assay and Transwell assay were used to determine the growth and invasion abilities of cancer cells.Results Luciferase intensity in As-miR-155 treated Lovo cells was significantly suppressed as compared with that in the control groups （P0.05）.The diameter of cultured clone in As-miR-155 treated Lovo cells was smaller than that in the control groups.Decreased cells via the transwell member in the AS-miR-155 treatment group were detected as compared with those in the control groups.Conclusion Over-expression of miR-155 induce the growth and invasion abilitiesof human colon cancer cell.

关 键 词：大肠癌 MIR-155 侵袭 

分 类 号：R735.34[医药卫生—肿瘤]