效应面法优化酶法提取大黄总蒽醌类成分的工艺研究  

Optimization of Enzymolysis Extraction of Total Anthraquinones from Wild Rhubarb (Rheum palmatum) Via Response Surface Methodology

作　　者：周天林[1] 井明博[1,2] 李东波[1] 焦飞[1] 

机构地区：[1]陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃庆阳745000 [2]陕西师范大学西北濒危药材资源开发国家工程实验室,陕西西安710062

出　　处：《中药材》2012年第10期1668-1674,共7页Journal of Chinese Medicinal Materials

基　　金：甘肃省庆阳市重大支撑项目(1003NKCM020);科技部国家科技支撑计划子课题(2006BA106A-04);中央高校基本科研业务费项目(GK201002080)

摘　　要：目的:优化酶法提取大黄总蒽醌工艺。方法:以大黄总蒽醌浓度为评价指标,用单因素方差分析法研究酶剂量、酶解温度、酶解时间和pH对提取大黄总蒽醌的影响;采用效应面法,以酶解大黄所提取的总蒽醌浓度为指标,对酶法提取大黄总蒽醌工艺条件进行优化。结果:酶剂量为0.12 g时,总蒽醌含量最高(3.1730±0.1023 mg/L),且大黄总蒽醌浓度随着酶解时间的增加而增加,并以酶解48 h蒽醌浓度最高(2.1410±0.1252 mg/L);此外,pH在4.8、酶解温度在50℃时,大黄总蒽醌浓度最高分别达到2.2777±0.2133 mg/L和4.1360±0.3877 mg/L。以效应面法优化酶法提取大黄总蒽醌的最佳工艺为:pH:4.59,酶剂量:0.1 g,酶解温度:45.86℃。结论:该工艺条件易于控制、工艺简单,成本低,在此条件下大黄酶解后总蒽醌的最高浓度为4.3231 mg/L。Objective：To optimize the technology of enzymolysis extraction of total anthraquinones.Methods： Used the total anthraquinone concentration extracted from rhubarb（Rheum palmatum,L） via enzymolysis as experimental assess index,effect of enzyme dose,enzyme solution temperature,enzyme solution time and pH on total anthraquinone extraction were investigated respectively based on single factor variance analysis.Furthermore,its extraction process was optimized based on response surface methodology.Results：The yield of total anthraquinones demonstrated the top（3.1730±0.1023 mg/L） while the enzyme dose was up to 0.12 g,besides,for the enzyme solution time,the total anthraquinones yield exhibited a trend of increase with the increase of enzyme solution time and the top concentration of total anthraquinones was up to 2.1410±0.1252 mg/L while it was 48 h;Moreover,the top field of total anthraquinones were up to 2.2777±0.2133 mg/L and 4.1360±0.3877 mg/L while the pH and enzyme solution temperature were up to 4.8 and 50 ℃ in respectively.Finally,the most optimum enzymolysis extraction processing technology via response surface methodology was as follows： pH： 4.59,enzyme dose： 0.1 g as well as enzyme solution temperature： 45.86 ℃.Conclusion： The condition of extraction processing technology is easily-controlled,simple and lower cost,and the final extraction concentration of total anthraquinone is up to 4.3231 mg/L under the processing technology above.

关 键 词：效应面法 酶法 大黄 总蒽醌 

分 类 号：R284.2[医药卫生—中药学]