Proteomic Identification of Rhythmic Proteins in Maize Seedling Leaves  

作　　者：FENG Wan-jun GUO Bao-jian YAO Ying-yin PENG Hui-ru SUN Qi-xin NI Zhong-fu 

机构地区：[1]Key Laboratory of Crop Heterosis and Utilization,Ministry of Education/State Key Laboratory for Agrobiotechnology,Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/College of Agriculture and Biotechnology, China Agricultural University [2]National Plant Gene Research Centre

出　　处：《Journal of Integrative Agriculture》2012年第12期1958-1967,共10页农业科学学报（英文版）

基　　金：supported by the National Basic Research Program of China(2007CB109000);the National Science Found for Distinguished Young Scholars, China(30925023);the National Natural Science Foundation of China(30671297);the National High-Tech R&D Program of China(2009AA101102)

摘　　要：Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.

关 键 词：PROTEOMICS rhythmic proteins maize leaves mass spectrometry two-dimensional gel electrophoresis 

分 类 号：S513[农业科学—作物学]