HPLC法检测降血糖保健食品中的双胍类药物  

Determination of Biguanides in Anti-diabetic Health Foods by HPLC

作　　者：王俊苏[1] 郗存显[1] 陈冬东[2] 张雷[1] 李贤良[1] 彭涛[2] 王国民[1] 

机构地区：[1]重庆出入境检验检疫局/重庆市进出口食品安全工程技术研究中心,重庆400020 [2]中国检验检疫科学研究院,北京100123

出　　处：《中国药房》2013年第1期68-71,共4页China Pharmacy

基　　金：2010年质检公益性行业科研专项资助(No.201010073);2009年国家质检总局科技计划项目资助(No.2009IK313);2009年重庆市科委项目资助(No.CSTC2009CB1012)

摘　　要：目的:建立同时检测降血糖保健食品中二甲双胍和苯乙双胍含量的方法。方法:采用甲醇-乙醇(50:50,V/V)超声提取降血糖保健食品降糖粉、奶粉、口服液、胶囊、清渴茶中的二甲双胍和苯乙双胍,提取液经WCX固相萃取柱净化,高效液相色谱法测定,基质回收标准曲线定量。结果:二甲双胍和苯乙双胍检测质量浓度线性范围均为0.1～5.0mg/L(r分别为0.9999、0.9995),回收率分别为91.9%～102.7%、89.8%～107.0%,RSD均低于9.46%,定量限均为0.1μg/g;5种试样中均未检出二甲双胍和苯乙双胍。结论:本方法简单、可靠、灵敏,可用于降血糖保健食品中双胍类药物的检测。OBJECTIVE：To establish the method for simultaneous determination of metformin and phenformin in anti-diabetic heath foods.METHODS：Metformin and phenformin were extracted from anti-diabetic heath foods ultrasonically with methanol-ethanol（50：50,V/V）,including anti-diabetic powder,milk powder,oral liquid,capsule and diabetes tea.The extraction liquid was cleaned up with WCX solid-phase extraction column,and determined by HPLC.Quantification was done by matrix-matched standard curve.RESULTS：The linear range of metformin and phenformin were 0.1-5.0mg/L（r=0.999 9,r=0.999 5）.Average recoveries of them were 91.9%-102.7% and 89.8%-107.0%,respectively（RSD9.46%）.The limits of quantification（LOQ）of them were 0.1 μg/g.None of metformin and phenformin was found in 5 samples.CONCLUSION：The method is simple,reliable and sensitive,which can be used to monitor biguanides in anti-diabetic health foods.

关 键 词：二甲双胍 苯乙双胍 保健食品 高效液相色谱法 检测 

分 类 号：R927.2[医药卫生—药学] R977.15