CPA与TNF-α检测在类风湿性关节炎中医辨证分型中的意义  

Significance of CPA and TNF-α detection on syndrome differentiation of patients with rheumatoid arthritis

作　　者：于秀明[1] 胡维峰[2] 

机构地区：[1]长春中医药大学附属医院免疫研究室,吉林长春130000 [2]吉林大学第一医院供应科,吉林长春130000

出　　处：《中国当代医药》2012年第36期99-100,共2页China Modern Medicine

摘　　要：目的将检测类风湿关节炎(RA)的实验室指标与中医对类风湿关节炎临床辨证分型相结合,为中医临床诊断客观化提供参考。方法选取本院2010年11月～2012年8月收治的RA患者200例,根据中医辨证分型将其分为5组,分别为寒湿痹阻型、湿热痹阻型、肝肾阴虚型、肾气虚寒型、瘀血痹阻型。采用酶联免疫吸附实验法(ELISA)检测血清CPA,放射免疫法(RIA)检测肿瘤坏死因子-α(TNF-α),比较各组患者间的检测结果。结果 200例患者行CPA检测,湿热痹阻型CPA阳性率明显高于寒湿痹阻型、肝肾阴虚型,差异均有统计学意义(P<0.05)。186例患者行TNF-α检测,寒湿痹阻型TNF-α含量明显高于肝肾阴虚型、瘀血痹阻型,差异均有统计学意义(P<0.05)。结论 CPA与TNF-α检测在类风湿性关节炎中医辨证分型中具有重要的临床价值,可为疾病的诊断提供有效的科学依据。Objective To provide a objective reference on traditional Chinese medicine（TCM） diagnosis of rheumatoid arthritis（RA） by combining TCM syndrome patterns of RA with objective indexes.Methods Two hundred RA patients were selected in the hospital from November 2010 to August 2012.According to the traditional Chinese medicine syndrome typing,patients were divided into 5 groups.There were Han-Shi-Bi-Zu group,Shi-Re-Bi-Zu group,Gan-Shen-Yin-xu group,Shen-Qi-Xu-Han group,Yu-Xue-Bi-Zu group.Serum CPA were detected by enzyme linked immunosorbent assay（ELISA）.Tumor necrosis factor-α（TNF-α） were detected by radioimmunoassay（RIA）.Test results were compared between groups.Results Two hundred patients had done CPA detection.CPA positive rate in Shi-Re-Bi-Zu group was significantly higher than that in Han-Shi-Bi-Zu group and Gan-shen-Yin-Xu group.The difference was statistically significant（P 〈0.05）.186 patients had done TNF-α detection.TNF-α content in Han-Shi-Bi-Zu group was significantly higher than that in Gan-shen-Yin-Xu group and Yu-Xue-Bi-Zu group.The difference was statistically significant（P 〈0.05）.Conclusion CPA and TNF-α detection has important clinical value in TCM syndrome differentiation of patients with rheumatoid arthritis,which can also provide the science basis for the diagnosis of diseases.

关 键 词：类风湿关节炎 辨证分型 抗瓜氨酸合成蛋白抗体 肿瘤坏死因子-Α 痹证 

分 类 号：R593.22[医药卫生—内科学]