胃腺癌cagA^+幽门螺杆菌感染中HIF-1α和iNOS的表达及意义  

Significance of HIF-1α and iNOS expression associated with cagA+ Helicobacter pylori infection in gastric adenocarcinoma

作　　者：张志广[1] 李熳[1] 闻淑军[1] 江勇[1] 翟浩宇[1] 季英兰[1] 

机构地区：[1]天津医科大学第二医院消化科,天津300211

出　　处：《天津医科大学学报》2012年第4期441-444,共4页Journal of Tianjin Medical University

摘　　要：目的:研究HIF-1α和iNOS在cagA+幽门螺杆菌(Hp)感染的胃腺癌组织中的表达。方法:收集临床病理资料完整的胃腺癌病历及蜡块138例,均已经c14呼气试验证实为Hp阳性,采用PCR方法检测胃腺癌石蜡组织中的cagA基因,采用免疫组织化学法检测HIF-1α和iNOS的基因表达。结果:cagA阳性表达与胃腺癌的浸润深度密切相关(P<0.05)。HIF-1α和iNOS在cagA+组中表达明显高于cagA-组(P<0.05)。结论:cagA基因可通过促进HIF-1α和iNOS的表达促进胃癌的发生。Objective： To investigate the expression of HIF Helicobacter pylori; gastric adenocarcinoma; hypoxia-inducible factor-1 ; inducible nitric oxide synthase; cytotoxin associ- ated protein and iNOS in cagA＋ Helicobacter pylori （Hp） infectious gastric adenocar- cinoma（GAC）. Methods： 138 paraffin-embedded human GAC samples, with detailed follow-up and clinicopathologic data were collected which had been identified as Hp-positively by c14 breath test in before. Polymerase chain reaction （PCR） was used to detect the cagA gene and immunohistochemical staining to detect the expression of HIF-1α and iNOS. Results： CagA＋ Hp infection was associated with invasive depth of GAC （P〈0.05）.Overexpression of HIF-1α and iNOS were seen in cagA＋ Hp GAC group （P〈0.05）. Conclusion： The carcinogenesis of cagA＋ Hp might be through the pathway of HIF-1α and iNOS.

关 键 词：幽门螺杆菌 胃腺癌 缺氧诱导因子-1 诱导型一氧化氮合酶 细胞毒素相关蛋白 

分 类 号：R735.2[医药卫生—肿瘤]