采用反复照射方法建立放射抗拒性鼻咽癌细胞系亚株  

Establishment of radioresistant subline from human nasopharyngeal carcinoma cell line by repeating irradiation

作　　者：彭露杏[1] 陈甲信[1] 程金建[1] 刘斐[2] 焦伟[2] 庞强[1] 冯国生[1] 李善观[1] 莫晓云[2] 伍欣星[3] 

机构地区：[1]广西壮族自治区人民医院临床肿瘤中心放疗科,南宁530021 [2]广西壮族自治区人民医院科研实验中心,南宁530021 [3]武汉大学医学院病毒学研究所分子病毒学研究室,湖北430071

出　　处：《中国临床新医学》2012年第12期1107-1109,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西自然科学基金资助项目(编号:桂科青0542041)

摘　　要：目的为成功建立放射抗拒性鼻咽癌细胞系亚株,并探讨鼻咽癌放射抗拒机理。方法采用类似临床放疗的照射模式反复照射从人鼻咽癌细胞系CNE-3中分离出具有放射抗拒性的新细胞系亚株。结果采用类似临床放疗的照射模式反复照射分离出具有放射抗拒性的新细胞系亚株CNE-3R,,其在传代3个月后仍表现出较稳定的放射抗拒性,其SF2值均>0.5。结论采用该研究方法能成功建立放射抗拒性鼻咽癌细胞系亚株,为研究鼻咽癌放射抗拒机理提供了必要的工具。Objective To establish radioresistant cell subline from a human nasopharyngeal carcinoma cell line CNE-3 and investigate the mechanism of radioresistanee. Methods Radioresistant cell subline were established from a human nasopharyngeal carcinoma cell line CNE-3 by repeating X-ray irradiation as radiotherapy. The radiosen- sitivity of this subline, together with its parent CNE-3 cell line, were measured. Results This subline, named CNE-3R could be subcultured steadily three months, and kept SF2 〉 0.5. Conclusion Radioresistant cell subline from a hu- man nasopharyngeal carcinoma cell line successfully was established by using the method of research, which providing the tools for the study of the mechanism of radioresistance.

关 键 词：反复照射 鼻咽癌 放射抗拒性 细胞系亚株 

分 类 号：R739.62[医药卫生—肿瘤]