促红细胞生成素对缺氧缺血新生鼠脑组织丙二醛水平及细胞凋亡的影响  

Effect of erythropoietin on MDA and neuronal apoptosis in brain of neonatal rats with hypoxia-ischemic brain damage

作　　者：张秋月[1] 陈国萍[1] 韩钢[1] 米延[1] 李丽[2] 

机构地区：[1]哈尔滨医科大学附属第一医院新生儿科,黑龙江哈尔滨150001 [2]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2012年第6期1-2,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目;编号:11531161

摘　　要：目的:探讨促红细胞生成素对新生鼠缺氧缺血性脑损伤的保护作用机制。方法:选用7日龄Wistar大鼠54只,分成假手术组、生理盐水对照组及促红细胞生成素治疗组,制备缺氧缺血性脑损伤(HIBD)模型后分别给予不同的处置于24h、48h、72h处死。检测脑组织中丙二醛(MDA),观察神经细胞凋亡情况。结果:盐水对照组MDA水平高、细胞凋亡指数高,同假手术组比较有显著性差异,促红细胞生成素治疗组MDA水平低、细胞凋亡指数低,同盐水对照组比较有显著性差异。结论:促红细胞生成素可抑制丙二醛水平增加及神经细胞凋亡,这可能是其对缺氧缺血性脑损伤的保护作用机制之一。Objective： Investigation of the protective mechanism of erythropoietin （EPO） on hypoxic--is- chemic brain damage of neonatal rats. Methods： Fifty--four wistar rats at the age of 7--day--old were divided into three groups,including normal group, HIBD groups, and EPO treatment group. After we established the model of HIBD, rats were killed at 24h, 48h, and 72h respectively. Then the apoptosis index （AI） was ob- served and the content of malondialdehyde （MDA） in brain tissues were detected correspondingly. Results. Higher MDA content and AI were shown in HIBD group when compared with normal group. Moreover, there was significantly lower MDA content and AI appeared in EPO treatment on normal group than that in HIBD group. Conclusion： Erythropoietin can reduce the level of MDA and AI, which may be one of protective mech- anisms on hypoxic--ischemic brain damage.

关 键 词：促红细胞生成素 缺氧缺血性脑损伤 丙二醛 细胞凋亡 

分 类 号：Q255[生物学—细胞生物学] R743[医药卫生—神经病学与精神病学]