高压氧对脑内种植GL261胶质瘤C57小鼠尼莫司汀化疗后生存期的影响  

The effect of hyperbaric oxygen on survival time of C57 mice implanted with GL261 gliomas after chemotherapy with ACNU

作　　者：兰川[1] 李飞[1] 陈图南[1] 叶信珍[1] 吴南[1] 易良[1] 林江凯[1] 朱刚[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科

出　　处：《中国现代神经疾病杂志》2012年第6期707-711,共5页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：国家自然科学基金资助项目(项目编号:30901538);国家自然科学基金资助项目(项目编号:30973494)~~

摘　　要：目的观察高压氧辅助治疗对脑内种植GL261胶质瘤细胞系C57小鼠尼莫司汀(嘧啶亚硝脲)化疗后平均生存期的影响。方法于C57小鼠右侧尾状核种植GL261胶质瘤细胞,术后第7天分别接受单纯高压氧或单次尼莫司汀化疗或高压氧与尼莫司汀联合治疗,观察不同治疗方法对荷瘤鼠生存期的影响。结果大体标本观察显示,不同处理组荷瘤鼠肿瘤体积均较大、占位效应明显,正常脑组织受压严重、中线结构偏移,但不同处理组肿瘤体积比较差异无统计学意义(F=0.602,P=0.618)。平均生存期(F=12.177,P=0.000)评价和Kaplan-Meier生存曲线分析(χ2=13.604,P=0.003)显示,单纯高压氧治疗(χ2=0.365,P=0.546)和单次尼莫司汀化疗(χ2=0.884,P=0.347)对荷瘤鼠生存期无明显影响;高压氧联合尼莫司汀化疗(χ2=9.962,P=0.002)可延长荷瘤鼠生存时间,且疗效优于单纯高压氧治疗(χ2=6.925,P=0.008)和单次尼莫司汀化疗(χ2=7.152,P=0.007)。结论高压氧对颅内种植GL261胶质瘤细胞系的C57小鼠生存期和生长无明显影响和促进作用,联合尼莫司汀化疗则可使荷瘤鼠平均生存期明显延长,提示高压氧对尼莫司汀化疗具有增效作用。Objective To investigate the effect of hyperbaric oxygen （HBO） on the survival of C57 mice implanted with GL261 gliomas after chemotherapy with nimustine （ACNU）. Methods Forty adult male C57 mice were divided into four groups： control, HBO, ACNU and HBO ＋ ACNU randomly by SPSS 10, and planted with GL261 glioma cell suspension in their caudate nucleus to form tumors. From the 7th day after implantation, these groups （except control group） began to receive different treatments （HBO, ACNU and HBO ＋ ACNU）, in order to observe the effects of different treatments on the survival time of tumor bearing mice. Results There were no significant differences in the tumor volume, mass effect, severe compression of normal brain tissue and midline shift （F = 0.602, P = 0.618）. The average survival time was significant with one-way ANOVA （F = 12.177, P = 0.000） and with the Kaplan-Meier survival analysis of log-rank （χ2= 13.604, P = 0.003）, while multiple comparisons between groups showed HBO （χ2= 0.365, P = 0.546） and single-time ACNU （χ2= 0.884, P = 0.347） had no effect on the survival of mice; the effect of HBO ＋ ACNU （χ2 = 9.962, P = 0.002） was better than that of HBO （χ2 = 6.925, P = 0.008） and singletime ACNU （χ2= 7.152, P = 0.007）. Conclusion The HBO had no effect on survival time of C57 mice with implantation of GL261 and could not promote the growth of the GL261 glioma. However, combining the treatment with ACNU could extend the survival time of tumor bearing mice, suggesting that HBO could strengthen the therapeutic effects of ACNU.

关 键 词：高压氧 神经胶质瘤 尼莫司汀 肿瘤种植 存活率 

分 类 号：R739.4[医药卫生—肿瘤]