中药提取物诱导血液肿瘤细胞凋亡的初步研究  

Study on CHP-induced cell apoptosis in hematopoietic malignancies

作　　者：夏迪[1] 贾培敏[1] 马瑜珊[1] 童建华[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院上海血液学研究所,上海200025

出　　处：《诊断学理论与实践》2012年第5期502-506,共5页Journal of Diagnostics Concepts & Practice

基　　金：国家自然科学基金(30971275和81170508);上海市领军人才"国家队"专项资金;上海市科委优秀学术带头人计划项目(11XD1403500)

摘　　要：目的:观察中药提取物(CHP)对急性髓系白血病细胞Kasumi-1和滤泡状淋巴瘤细胞Su-DHL-4的诱导凋亡效应。方法:将浓度为50μg/mL的CHP分别作用于Kasumi-1和Su-DHL-4细胞,观察细胞的生长状况与形态变化;应用流式细胞术检测磷脂酰丝氨酸的外翻、线粒体跨膜电位及凋亡抑制蛋白Bcl-2的表达变化情况。结果:Kasumi-1和Su-DHL-4细胞经50μg/mL CHP处理后,生长均受到抑制,48 h时的生长抑制率分别为(69.45±7.21)%和(82.44±5.86)%,并可观察到典型的凋亡细胞。与对照组相比,CHP处理24 h后,2种细胞的AnnexinⅤ阳性率分别从(10.58±1.22)%和(4.30±0.52)%上升到(53.53.±6.07)%和(59.87±5.56)%,差异均有统计学意义(P<0.01);线粒体跨膜电位下降的细胞百分率从(6.67±0.64)%和(7.20±2.33)%上升到(31.13±1.80)%和(55.97±7.33)%,差异亦有统计学意义(P<0.01);此外,Su-DHL-4细胞中Bcl-2蛋白水平也有明显下降。结论:CHP能诱导急性髓系白血病细胞Kasumi-1和滤泡性淋巴瘤细胞Su-DHL-4发生凋亡。Objective： To study the effect of CHP （a Chinese traditional medicine extract） on apoptosis of acute myeloid leukemia cell line Kasumi-1 and follicular lymphoma cell line Su-DHL-4. Methods： Kasumi-1 cells and Su-DHL-4 cells were treated respectively with CHP at the concentration of 50 μg/mL. The effect of CHP was evaluated by cell growth, cell morphology and markers of cell apoptosis. The plasma membrane translocation of phosphatidylserine, the mitochondrial transmembrane potential, and the expression of anti-apoptotic protein Bcl-2 were detected by flow cytometry. Results： CHP inhibited significantly the cell proliferation of Kasumi-I cells and Su-DHL-4 cells. After 48 h treatment with CHP, the rates of growth inhibition of Kasumi-1 and Su-DHL-4 cells were （69.45＋7.21）% and （82.44＋5.86）%, respectively, and typical apoptotic cells could be observed. In addition, compared with the two control groups, the percentage of Annexin V positive cell in these two cell lines treated with CHP for 24h were raised significantly from （10.58±1.22）% and （4.30±0.52）% to （ 53.53±6.07）% and （59.87±5.56）%, respectively （P〈0.01）. Meanwhile, the mitoehondrial transmembrane potential of these two eel1 lines increased markedly from （6.67±0.64）% and （7.20±2.33）% to （31.13±1.80）% and （55.97±7.33）% （P〈0.01）. In addition, the expression of Bcl-2 in Su-DHL-4 cells was obviously down-regulated. Coneluslons： The traditional Chinese medicine extract CHP could induce apoptosis in both Kasumi-1 cells and Su-DHL-4 cells.

关 键 词：细胞凋亡 中药提取物 流式细胞术 

分 类 号：R733.4[医药卫生—肿瘤]