重编程相关因子在牙髓和牙周组织损伤修复中的表达和作用  

The expression and modulatory role of reprogramming markers on the in vivo regeneration of dental pulp and periodontal ligament tissue after injury

作　　者：刘路[1] 韦曦[1] 凌均棨[1] 彭正军[1] 吴莉萍[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院·广东省口腔医学重点实验室.广东广州510055

出　　处：《上海口腔医学》2012年第6期601-605,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81070830);中央高校基本科研业务费专项资金(11ykpy51);广东省医学科研基金(B2012141);广东省中医药局科研课题(20121147);广东省科技计划项目(2010B031600063)~~

摘　　要：目的:探讨重编程相关因子Oct-4、Sox2和c-Myc在大鼠牙髓组织和牙周韧带组织损伤修复过程中的表达及作用。方法:建立大鼠牙髓牙周联合损伤动物模型,HE染色观察4周后牙髓牙本质复合体及牙周附着装置修复情况,免疫组织荧光染色检测重编程相关因子Oct-4、Sox2和c-Myc在牙髓和牙周联合损伤修复动物体内模型的表达和分布。结果:HE染色显示,大鼠牙髓牙周联合损伤4周后,新生的牙髓牙本质复合体及牙周附着装置形成;免疫荧光显示,在体内牙髓牙周组织损伤修复的过程中,Oct-4、Sox2和c-Myc在牙髓及牙周损伤修复的新生组织均明显激活,Oct-4和Sox2呈现相似的表达趋势。结论:Oct-4、Sox2和c-Myc均参与体内牙髓牙本质复合体和牙周附着装置的损伤修复,Oct-4和Sox2呈现相似的表达趋势,提示Sox2和Oct-4可能存在协同调控作用,而c-Myc可能具备独立的功能。PURPOSE： The purpose of this study was to investigate the expression of reprogramming markers including Oct-4, Sox2 and e-Myc in rat dental pulp and periodontal ligament tissues undergoing tissue regeneration after injury. METHODS： Lewis rat model with dental pulp and periodontal ligament injury was created. HE staining was applied to investigate the structure of newly formed dentin-pulp complex and periodontium. The distribution of Oct-4, Sox2 and c- Mye was determined by immunofluorescent staining. RESULTS： HE staining showed that newly formed dentin-pulp complex and periodontium was constructed 4 weeks after injury. Immunofluorescent staining revealed Oct-4, Sox2 and c- Myc signal was activated in the newly formed dental pulp and periodontal ligament tissue after injury compared with the normal ones. Oct-4 and Sox2 showed similar expression pattern. CONCLUSIONS： Oct-4, Sox2 and c-Myc signaling may play an important modulatory role in the dental tissue regeneration after injury. Oct-4 and Sox2 may have operative interaction whereas c-Myc may play independent role during this process. Supported by National Natural Science Foundation of China （81070830）, Fundamental Research Funds for the Central Universities （llykpyS1）, Department of Health of Guangdong Province （B2012141）, Bureau of Chinese Medicine of Guangdong Province （20121147） and Bureau of Science and Technology of Guangdong Province （2010B031600063）.

关 键 词：牙髓组织 牙周组织 重编程相关因子 损伤修复 

分 类 号：R781[医药卫生—口腔医学]