大鼠正畸牙移动过程中牙周组织内CGRP的表达变化  

The change of calcitonin gene-related peptide in periodontal tissue during orthodontic tooth movement

作　　者：乔虎[1] 周洪[1] 朱永进 高宇男[1] 

机构地区：[1]西安交通大学医学院附属口腔医院正畸科 [2]口腔医学研究中心,陕西西安710004

出　　处：《上海口腔医学》2012年第6期606-610,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30973359);陕西省自然科学基金(2009JQ4007)~~

摘　　要：目的:建立SD大鼠正畸牙移动模型,观察牙移动过程中牙周组织内降钙素基因相关肽(calcitonin gene related peptide,CGRP)的表达变化,探讨其在正畸疼痛的外周神经机制中的作用。方法:将60只SD大鼠随机分为实验组(54只)和对照组(6只)2组,实验组大鼠施加50 g力后,分别在4 h、8 h、1 d(1 d组根据据力值大小分为1 d-30 g、1 d-50 g、1 d-80 g 3个亚组)、3 d、5 d、1周、2周,随机处死6只SD大鼠,收集牙周组织,进行实时定量PCR检测,观察牙周膜内CGRP随时间及力值大小的表达变化,采用SPSS13.0软件包对数据进行统计学分析。结果:大鼠正畸牙移动过程中,牙周组织内CGRP表达随加力时间发生改变,加力4 h后开始增强,1 d后达到高峰并逐渐减弱,2周后与对照组相比无显著差异。不同力值组加力1 d后,CGRP表达随加力力值增大而升高,80 g力组>50 g力组>30 g力组。结论:正畸牙移动过程中,牙周组织内CGRP表达随加力时间增加及加力力值增大出现规律性变化,提示CGRP可能在正畸疼痛的外周神经机制中具有重要作用。PURPOSE： To establish a Sprague-Dawley rat model of tooth movement, detect the expression of calcitonin gene-related peptide （ealeitonin gene related peptide, CGRP） in Sprague-Dawley rats＇ periodontal tissues during tooth movement and explore the role of the peripheral mechanisms of pain in orthodontics. METHODS： 60 Sprague-Dawley rats were randomly divided into experimental group （n=54） and control group （n=6）. Force was applied on right maxillary first molar in rats and the changes of CGRP expression were detected by real-time PCR 4 h, 8 h, 1 d, 3 d, 5 d, 7 d, 14 d after tooth movement. SPSS 13.0 software package was selected and one-way ANOVA （least significant difference, LSD） was used for statistics analysis. RESULTS： Experimental tooth movement led to an increase in the expression of CGRP. In addition, following experimental tooth movement, the expression of CGRP in periodontal tissues was significantly up- regulated from 4 h, with a peak on 1 d and returned to the control level at 2-week. 1 d after experimental tooth movement, the application of different forces to rats induced a significant increase in CGRP expression. CONCLUSION： The regular change of CGRP expression in periodontal tissues indicates that CGRP may play an important role in peripheral mechanism of orthodontic pain. Supported by National Natural Science Foundation of China （30973359） and Natural Science Foundation of Shanxi Province （2009J04007）.

关 键 词：降钙素基因相关肽 实时定量聚合酶链反应 正畸疼痛 

分 类 号：R783.5[医药卫生—口腔医学]