母爱剥夺诱导的抑郁大鼠海马miR-16的表达  

Expression of miR-16 in the hippocampus of depressed adult rats induced by maternal deprivation

作　　者：张逸[1] 朱熊兆[1] 张晟[1] 白玫[1] 张丽[1] 薛亮[1] 

机构地区：[1]中南大学湘雅二医院医学心理学研究所,长沙410011

出　　处：《中南大学学报（医学版）》2012年第12期1193-1197,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81071097)~~

摘　　要：目的:研究母爱剥夺是否诱发成年大鼠抑郁样行为表现,miR-16是否参与母爱剥夺诱发抑郁的病理过程。方法:在SD大鼠出生后第1天按窝随机分为母爱剥夺组(n=17)和正常对照组(n=17)。母爱剥夺组大鼠在出生后第1至14天每天接受6 h的母爱剥夺应激,正常对照组大鼠不接受任何实验处理。在大鼠13周龄时采用强迫游泳和糖水偏爱实验评定大鼠的抑郁水平;采用实时荧光定量RT-PCR检测大鼠海马miR-16的表达水平。结果:在强迫游泳实验中,母爱剥夺组大鼠的被动漂浮时间显著长于对照组大鼠,糖水偏爱率显著低于对照组(P<0.05);与对照组比较,母爱剥夺组大鼠海马内miR-16的表达水平显著增加(P<0.05),miR-16表达水平与抑郁样行为水平显著相关(P<0.05)。结论:母爱剥夺能使大鼠成年后表现出抑郁样行为,海马内miR-16表达水平升高,miR-16可能参与母爱剥夺诱发大鼠抑郁样行为的病理过程。Objective： To detect the expression of miR-16 in the hippocampus of a rat depression model induced by maternal deprivation,and to explore whether miR-16 is involved in the pathological process of maternal deprivation-induced depression.Methods： Newborn SD rats were randomly divided into a maternal deprivation group（n=17） and a control group（n=17）.Rats in the maternal deprivation group experienced maternal deprivation for 6 h per day from 1st to 14th postnatal day,while rats in the control group rats received no treatment.When the rats were 13 weeks old,depression-like behaviors were assessed by forced swimming test and sucrose consumption test,and the expression of hippocampal miR-16 in rats was detected by real-time RT-PCR.Results： Maternal-deprived rats exhibited significantly longer passive floating time and lower sucrose preference rate than rats in the control group（P0.05）.Maternal-deprivation rats expressed higher level of miR-16 in the hippocampus than rats in the control group,and the expression level of miR-16 was significantly associated with the passive floating time（r=0.65,P0.05） and the sucrosepreference rate（r=–0.59,P0.05）.Conclusion： Maternal deprivation can induce depressive behaviors in rats and increase the expression of miR-16 in the hippocampus in rats.MiR-16 may be involved in the pathological mechanism of the maternal deprivation-induced depression.

关 键 词：母爱剥夺 抑郁 海马 miR-16 

分 类 号：R749.4[医药卫生—神经病学与精神病学]