复方苦参水溶液体外对人结肠癌细胞SW480的影响  

An in vitro study of effect of Kushen compound preparation on human colon cancer cell line SW480

作　　者：周林[1] 艾中立[1] 刘志苏[1] 钱群[1] 何跃明[1] 

机构地区：[1]湖北医科大学附属第二医院普通外科,湖北武汉430071

出　　处：《癌症》2000年第4期337-340,共4页Chinese Journal of Cancer

摘　　要：目的 :探讨复方苦参水溶液体外对结肠癌细胞的影响。方法 :应用MTT法 ,生长曲线 ,荧光染色 ,流式细胞仪 ,透射电镜技术研究复方苦参水溶液体外对SW 480细胞的增殖抑制及诱导凋亡作用。结果 :复方苦参水溶液体外对SW 480细胞有明显的抑制作用 ,而且这种抑制作用呈现浓度和时间的依赖性。 30mg/ml中药作用SW 480细胞 48小时后 ,光镜及电镜下可见胞核固缩 ,荧光染色增强 ,胞核碎裂 ,凋亡小体形成等凋亡形态学变化。流式细胞仪检测到亚G1峰 ,其凋亡率为 11 13%。细胞周期分析发现 ,经 30mg/ml药物处理 48小时后 ,S期细胞由对照组的 2 5 41%上升到 41 2 7% (P <0 0 1) ,G2 /M期细胞由 6 74%降至 1 35 % (P <0 0 1)。结论 :复方苦参水溶液体外能够抑制SW 480细胞增殖并且在一定浓度范围内诱导其凋亡 ,诱导凋亡可能是其发挥抗肿瘤作用的重要机制之一。Objective: To explore the effect of Kushen compound preparation on human cancer cell line SW480 in vitro Methods: Inhibition of SW480 cell growth by Kushen compound preparation was determined by MTT assay and growth curve, and apoptosis was detected by morphological assay and flow cytometry (FCM) Results: The solution of Kushen compound preparation showed dose dependent and time dependent inhibitory effects on the proliferation in SW480 cell line The cells treated with 30 mg/ml compoumd for 48 hours showed characters of apoptosis under light and electronic microscopy Sub G 1 peak was detected by FCM, the ratio of apoptosis was 11 13% The cell percent of S phrase increased from 25 41%to 41 27%(P< 0 01), and that of G 2/M phrase decreased from 6 74%to 1 35%(P< 0 01) after treatment Conclusion: In vitro, Kushen compound preparation has growth inhibiting effect and may induce apoptosis in SW480 in a certain dose range, which may be one of the antineoplastic effects of Kushen compound preparation

关 键 词：结肠肿瘤 复方苦参 细胞凋亡 中医药疗法 

分 类 号：R735.35[医药卫生—肿瘤]