4种植物精油的抑菌活性及应用  

Inhibitory Activities and Application of Four Plant Essential Oils

作　　者：邓业成[1] 宁蕾[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2012年第3期288-294,共7页Journal of Guangxi Normal University:Natural Science Edition

基　　金：广西科学研究与技术开发计划基金资助项目(10169-08);广西自然科学基金资助项目(桂科自0991097)

摘　　要：采用液体培养基稀释法测定4种植物精油对11种动物病原细菌的最低抑制浓度(MIC),山苍子精油、南丰蜜橘精油、飞蓬精油和胜红蓟精油的MIC分别为0.062 5～0.5、0.125～1、2～4和0.062 5～0.125mL/L;山苍子精油、南丰蜜橘精油和胜红蓟精油对供试病原细菌的抑菌活性好,飞蓬精油的抑菌活性相对较差。采用气态熏蒸法测定4种植物精油对环境常见霉菌的抑菌效果,当精油剂量为16μL/皿时,4种植物精油对环境霉菌有抑制作用,山苍子精油和南丰蜜橘精油的抑制效果较好(此2种精油在27°C下对面包霉变有较好的抑制效果)。通过对植物精油漱口水的配方研究,确定了配方中各成分的最佳配比,并制备出山苍子精油漱口水。用山苍子精油漱口水漱口,对口腔细菌的除菌率为39.96%,与市售漱口水的除菌率相似,在除菌方面达到了工业产品的要求。采用GC/MS技术鉴定了4种植物精油的主要化学成分。Inhibitory activities of four plant essential oils against eleven animal pathogenic bacteria and environmental mould were determined using broth dilution method and gaseous fumigation.The results showed that The MICs of oils from Litsea mollis,Citrus reticulata,Erigeron acer and Ageratum conyzoides to the tested animal pathogenic bacteria were 0.062 5～0.5,0.125～1,2～4 and 0.062 5～0.125 mL/L,respectively.The inhibitory activities of oils from L.mollis,C.reticulata and E.acer were high,and that of A.conyzoides oil was relatively low.At a dose of 16 μL per utensil,the four plant essential oils had inhibition against environmental mould,and the inhibitory activities of oils from L.mollis and C.reticulata were higher than that of another two oils.The oils of L.mollis and C.reticulata also had inhibitory effect on mould growth of bread at 27 °C and RH 80%.By studying the formulation of mouthwash of plant essential oil,the optimum proportion of all components in the mouthwash was confirmed,and the mouthwash of L.mollis essential oil prepared.The removing rate of mouth bacteria by the new mouthwash reached 39.96%,which was similar to the removing rate by commercial mouthwash and met the demand of industrial product.The principal compounds of four plant essential oils were also identified using GC/MS technique.

关 键 词：植物精油 动物病原细菌 抑菌活性 防腐 漱口水 

分 类 号：Q946.8[生物学—植物学]