SDF-1α促进BMSCs对脑缺血／再灌注大鼠CA1区神经元的保护作用  

Promotion of SDF -1α to the protective effect of BMSCs to the neurons in hippocampal CA1 area in cerebral ischemia/reperfusion rats

作　　者：王玉兰[1,2] 李爱娜[1] 张世春[1] 刘志安[1,2] 高殿帅[2] 

机构地区：[1]徐州医学院解剖学教研室,江苏徐州221004 [2]徐州医学院神经生物学教研室

出　　处：《徐州医学院学报》2012年第11期705-708,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（81171141）;徐州医学院院长专项人才基金（2010KJZ19）

摘　　要：目的观察基质衍生因子1α（SDF-1α）促进骨髓间充质干细胞（BMSCs）对脑缺血／再灌注大鼠CA1区神经元的保护作用。方法原代细胞培养BMSCs，传至第3代时以BrdU标记；以“四血管阻断”法制作大鼠脑缺血／再灌注模型，造模成功鼠随机分为模型对照组、BMSCs组和SDF-1α＋BMSCs组。后2组经尾静脉分别注射BMSCs或SDF1α＋BMSCs，模型对照组同法注射等量生理盐水，注射后分别存活1、3、7天和1个月，灌注固定制备石蜡切片，苏木精-伊红染色，光镜观察并计数各组大鼠海马CA1区锥体细胞在不同时间点的损伤表现。结果各组CA1区的形态异常细胞数高峰均出现于第3天，以后随时间延长而减少。但在各时间点内，BMSCs组和SDF-1α＋BMSCs组CAl区形态异常细胞数均明显低于模型对照组，且SDF-1α＋BMSCs组显著低于BM—SCs组，差异均有统计学意义（P〈0．05）。结论BMSCs移植可保护缺血／再灌注损伤大鼠海马CA1区的细胞损伤，且SDF-1α对这种保护有促进作用。Objective To investigate the protective effect of born nmrrow mesenchymal stem cells （BMSCs） promo- ted by stromal cell - derived factor -1α （ SDF -1α） to the nerve cells of hippocampal CA1 area cerebral ischemia/reper- fusion rats. Methods Cultured BMSCs and ＂four vessel occlusion＂ method were used for the cerebral I/R model. After reperfusion 24 h （hour）, the saline （NS）, BMSCs and SDF-1α＋ BMSCs were respectively transplanted into the L/R model by the tail vein, and immunofluorescence determination was employed for examining the BMSCs of migrating to hip- pocampus at different time points of BMSCs transplantation. The models were divided into three groups： sham group, BMSCs group and SDF -1α ＋ BMSCs group. The model animals were executed after transplanting 1, 3, 7 d and 1 M. Results The cell number of abnormal modality was a peak at 3 days after transplanting in each group , and it was de- creased following the time extended. In BMSCs group and SDF -1α ＋ BMSCs group, the cell number of abnormal modal- ity were lower than the sham group at the same time after transplanting, and the SDF - 1α ＋ BMSCs group was the lowest among three groups. The difference was statistically significant （ P 〈 0.05 ）. Conclusion The transplantation of BMSCs can improve cellular damage of hippocampal CAI area after ischemia/reperfusion, while SDF -1α can promote the protection.

关 键 词：骨髓间充质干细胞 基质衍生因子1α CA1 脑缺血 再灌注损伤 大鼠 

分 类 号：R364.12[医药卫生—病理学]