白血病相关原癌基因在氢醌处理TK6细胞中的表达  

Expressions of leukemia-related proto-oncogenes in hydroquinone-treated TK6 cells

作　　者：凌晓璇[1,2] 刘林华[3,2] 梁海荣[1,2] 戴娟秀[3,2] 郭莲仙[3,2] 唐焕文[3,2] 

机构地区：[1]广东医学院公共卫生学院实验中心,广东省东莞市523808 [2]广东医学院环境与健康研究所,广东省东莞市523808 [3]广东医学院公共卫生学院劳动卫生与环境卫生学教研室,广东省东莞市523808

出　　处：《职业与健康》2012年第24期3009-3011,共3页Occupation and Health

基　　金：国家自然科学基金资助(项目编号:30972500);广东省自然科学基金(项目编号:7301507);东莞市科技计划项目(项目编号:201010815206)

摘　　要：目的探索氢醌(hydroquinone,HQ)致DNA整体低甲基化的后续结果。方法用磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0、10.0和20.0μmol/L HQ染毒TK6细胞为处理组。应用实时荧光定量-聚合酶链反应检测白血病相关原癌基因MPL、BRAF、ERBB2、MYB、MYC和RAF1的表达水平。结果与对照细胞相比,HQ处理细胞MPL和RAF1的mRNA表达量都随HQ剂量增加而上升,其中20μmol/L HQ对原癌基因表达的影响最为明显,分别上升了80%(P<0.05)和35%(P<0.05);MYB、MYC和ERBB2基因的mRNA表达量随HQ的剂量上升而下降。结论 MPL的转录活化很有可能与HQ导致的DNA整体低甲基化有关。[ Objective ] To explore the consequences of global DNA hypomethylation induced by hydroquinone （HQ）. [ Methods ] HQ was dissolved with PBS buffer, 2.5, 5.0, 10.0 and 20.0 /μmol/L HQ were given to the TK6 cells respectively as treatment group. And the cells treated with PBS was defined as control group. Expressions of MPL, BRAF, ERBB2, MYB, MYC and RAF1 were measured by fluorescence quantitative RT-PCR assay. [ Results ] Expressions of MPL and RAF1 mRNA in the HQ-treated cells were increased with HQ dose increasing, comparing with the control cells, and 20 μmol/L HQ treatment affected the expres- sions of both genes significantly, increased by 80% （ P 〈 0.05 ） and 35% （ P 〈 0.05 ） , respectively. While the expressions of MYB, MYC and ERBB2 mRNA were decreased with the increasing of HQ concentrations. [ Conclusion ] MPL transcriptional activa- tion may be associated with global DNA hypomethylation induced by HQ.

关 键 词：氢醌 TK6细胞 原癌基因 MPL基因 

分 类 号：R733.7[医药卫生—肿瘤]