重组人白介素2-卡介苗对荷瘤小鼠腹腔巨噬细胞体外杀伤活性的影响  

Effect of Recombinant BCG Secreting Human IL-2 on Cytotoxicity of Murine Peritoneal Macrophages

作　　者：梅玉屏[1] 曾星[1] 黄羽 章国来 

机构地区：[1]广州中医药大学第二附属医院中心实验室,广州510120

出　　处：《中国肿瘤生物治疗杂志》2000年第1期32-34,共3页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金!(39870944);广东省自然科学基金博士启动基金!(97424)资助

摘　　要：目的：研究能分泌人白细胞介素２的重组卡介苗（ｒＢＣＣ－ＩＬ－２）对小鼠腹腔巨噬细胞体外杀伤活性功能的改变，探讨ｒＢＣＧ－ＩＬ－２对宿主免疫功能的影响。方法：采用基因工程技术和电转化技术构建卡介苗，采用ＭＴＴ法测定小鼠腹腔巨噬细胞体外的杀伤活性。结果：ｒＢＣＧ－ＩＬ－２组小鼠腹腔巨噬细胞杀伤活性明显大于ｒＢＣＧ组和对照组，且巨噬细胞的杀伤活性在一周内达到最大。结论：ｒＢＣＧ－ＩＬ－２能提高巨噬细胞的杀伤活性，是一种很有前景的生物制剂。Objective: To investigate the effect of recombinant BCG secreting IL-2 (rBCG-IL-2) on peritoneal macrophages of mouse and inquire into the effect of rBCG-IL-2 on immunity of host. Methods: Gene engineering technology, electricity transfer- ence and inoculation tumor cell are adopted; MTT assay is used to determine tumoricidal effect of macrophages. Results: Tumo- ricidal effect of macrophages of rBCG-IL-2 groups was higher than those of the control coups and BCG groups, and the tumorici- dal effect of macrophages could reach a high state in a week. Conclusion: rBCG-IL-2 can raise tumoricidal effect of macrophages, improve immunity of host and is a prospect biological preparation.

关 键 词：巨噬细胞 肿瘤 杀伤活性 rBCG-IL-2 小鼠 

分 类 号：R730.51[医药卫生—肿瘤] R392.11[医药卫生—临床医学]