融合基因pCEAcd-tk/前体药物体系的体外旁观者效应的差异性  

Variation of Bystander Effect of E coli. CD/HSV1-tk and Prodrugs System in vitro

作　　者：郭语彬[1] 伍新尧[2] 罗超权[1] 郭俊明[1] 许道松[1] 刘敏[3] 

机构地区：[1]中山医科大学生化教研室,广州510080 [2]中山医科大学法医学系 [3]中山医科大学第一附属医院中心实验室

出　　处：《中国肿瘤生物治疗杂志》2000年第1期42-45,共4页Chinese Journal of Cancer Biotherapy

基　　金：在读博士研究生项目!(331098)资助

摘　　要：目的：探讨融合自杀基因ｐＣＥＡｃｄ－ｔｋ在导入到肺癌细胞后，其体外旁观者效应的细胞差异性。方法：融合基因ｐＣＥＡｃｄ－ｔｋ提取后，脂质体介导的方法转染到６种肺癌细胞株（ＳＰＣＡ－１，ＧＬＣ－８２，Ａ５４９，ＳＣ，ＨＩＡＭＰ，Ｌ－７８），用ＭＴＴ法测定在细胞转染前后对前体药物５－氟胞嘧啶（５－ＦＣ）和丙氧鸟苷（ＧＣＶ）的敏感性和在体外的旁观者效应。结果：①脂质体介导的基因转染效率很低。②在６种已转染的肺癌细胞株中，有２种肺癌细胞株（ＳＰＣＡ－１，Ａ５４９）对前体药物５－ＦＣ和ＧＣＶ有较高的敏感性；③旁观者效应显示，２种细胞株（ＳＰＣＡ－１，ＳＣ）有强的体外旁观者效应，１种细胞株（Ａ５４９）有较强的旁观者效应，而另外３种细胞株（ＧＬＣ－８２，ＨＩＡＭＰ和Ｌ－７８）的旁观者效应较差。结论：不同的细胞在转染了融合自杀基因以后，其体外旁观者效应存在较大的差异。Objective: To investigate the variation in vitro bystander effect among human lung cancer cell lines aller transfection of pCEAcd-tk. Methods: pCEAcd-tk was extracted and transfected to six human lung cancer cell lines. Their sensitivity to 5- fluorocytosine (5-FC) and ganciclovir (GCV) was measured by MTT assay. Their bystander effect was also detendned. Re- sults: 1. The efficiency of lipid mediated gene transfer was very low; 2. Two out of six human lung cancer cell lines(SPCA-1, A549) have a high sensitivity to 5-FC and GCV; 3. Bystander effect: Two out of six human cancer cell lines(SPCA-1,SC) have a good bystander effect, and one out of them(A549) has a intermediate bystander effect, but three out of them (GM-82, HIAMP and L-78)have a poor bystander effect. Conclusion: There are a variation in vitro bystander effect in six human lung cancer cell lines after transfected with pCEAcd-tk.

关 键 词：旁观者效应 基因治疗 pCEAcd-tK 肺肿瘤 

分 类 号：R734.205[医药卫生—肿瘤] R730.51[医药卫生—临床医学]