抗PML-RARα反义核酸对NB4细胞生长、细胞周期及细胞凋亡的影响  

Effects of PML-RARα Antisence on the Growth, Cell Cycle and Apoptosis of NB4 Cells

作　　者：陈烨[1] 缪金明[1] 朱学宏[1] 邵念贤[1] 方智雯[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院血液科

出　　处：《中国肿瘤生物治疗杂志》2000年第1期56-59,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金!(39590291)资助

摘　　要：目的：探讨抗ＰＭＬ－ＲＡＲα反义核酸对早幼粒白血病细胞生长、细胞周期、细胞凋亡的作用。方法：以ＮＢ４细胞株为研究对象；细胞计数采用台盼兰排除、计数板计数法；白血病细胞集落采用甲基纤维素半固体培养；流式细胞仪（ＦＣＭ）用于检测细胞凋亡和细胞周期。结果：以６０μｇ／ｍｌ的终浓度处理细胞，抗 ＰＭＬ－ＲＡＲα融合部位反义核酸（ＦＵＡＳ）能明显抑制ＮＢ４细胞增殖及白血病细胞集落（ＡＭＬ－ＣＦＵ）形成，最大抑制率分别为４６．８％，５１．６％；ＧＭ－ＣＳＦ能减弱ＦＵＡＳ的作用，ＦＵＡＳ作用时间越短，ＧＭ－ＣＳＦ促ＡＭＬ－ＣＦＵ增加越明显；ＦＵＡＳ处理第７，９天，出现明显的凋亡细胞群，凋亡细胞百分比分别为４１．０％，３４．２％；ＦＵＡＳ处理第５天Ｓ期下降，Ｇ２／Ｍ期升高，第７天Ｃ０／Ｇ１期明显升高，Ｓ期回升。结论：抗ＰＭＬ－ＲＡＲα反义核酸具有抑制早幼粒白血病细胞增殖、诱导细胞凋亡的作用。Objective: To investigate the effects of PML-RARαantisense on the growth, cell cycle and apoptosis of NB4 cells. Methods: Cell apoptosis and cell cycle were detected by flow cytometry, and leukemic colony forming unit was exammed by methylcellulose assays. Results: PML-RARα antisence (FUAS) could inhibited the growth, and formation of acute myelocytic leukemia colony forming unit (AML-CFU)in NB4 cells; GM-CSF could enhance AML-CFU formation and antagonized the effect of FUAS on AML-CFU formation inhibition. The shorter the time of incubation with FUAS was, the more markedly GM-CSF en- hanced the AML-CFU formation. At 7 days, 9 days of treatment with FUAS percentage of apoptotic cells was 41. 5 %, 34. 2% respectively. Reduction of S phase and increase of G2/M Phase at 5 days, significant increase of G0/G1 Phase with S phase at 7 days. Conclusion: PMLRARα antisence can inhibit the cell growth and induce cell apoptosis of NB4 cells.

关 键 词：早幼粒细胞 急性白血病 反义核酸 PML-RARΑ 

分 类 号：R733.71[医药卫生—肿瘤]