分子生物学与形态学相结合鉴定盐生海芦笋内生真菌Salicorn 15  

Identification of Halophilic Endophytic Fungus Salicorn 15 Isolated from Salicornia bigelovii Using Molecular Biology Combined with Morphology

作　　者：曹娜[1] 湛东锐[1] 李连强[1] 王惠[1] 刘天行[1] 辛志宏[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2012年第23期180-183,共4页Food Science

基　　金：国家自然科学基金面上项目(31071586);2011年国家大学生创新训练计划项目(111030739);江苏高校优势学科建设工程项目

摘　　要：从盐生海芦笋中分离出一株内生真菌(Salicorn 15),采用分子生物学与形态学观察相结合对其鉴定。提取该菌的基因组DNA后分别对其18S rDNA和ITS区域进行PCR扩增并进行单克隆测序分析,在NCBI上进行同源性分析,进而构建系统发育树,再结合形态学观察,将菌株Salicorn 15鉴定为层出镰刀菌Fusarium proliferatum。An endophytic fungus designated as Salicorn 15 was isolated from halophilic Salicornia bigelovii and identified by molecular biology techniques combined with morphological observation.Genomic DNA from Salicorn 15 was extracted and its 18S rDNA and ITS region were amplified by PCR and sequenced for sequence homology analysis using NCBI BLAST.Based on phylogenetic analysis combined with morphological observation,Salicorn 15 was identified as Fusarium proliferatum.

关 键 词：海芦笋 18S RDNA ITS RDNA 鉴定 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]