有机溶剂稳定性蛋白酶发酵动力学研究  

Fermentation Kinetics of Organic Solvent-stable Protease Production

作　　者：房耀维[1] 余勃[2] 刘姝[1] 王淑军[1] 吕明生[1] 焦豫良[1] 曹纯[1] 

机构地区：[1]淮海工学院海洋学院江苏省海洋资源研究院,江苏连云港222005 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品科学》2012年第23期240-243,共4页Food Science

基　　金：江苏省高校自然科学基础研究面上项目(10KJB550002)

摘　　要：利用分批发酵研究海洋细菌Bacillus aquaemaris DS1分泌有机溶剂稳定性蛋白酶的代谢特性,结果表明蛋白酶合成和菌体生长呈部分生长关联型。据分批发酵实验结果采用Logistic方程、Luedeking-Piret方程建立了描述菌株生长、产酶以及底物消耗分批发酵动力学模型。动力学模型计算值与实验值拟合良好,相对误差小于10%。Metabolic characteristics and the synthesis of organic solvent-stable protease during batch fermentation of Bacillus aquaemaris DS1 were investigated.The production of organic solvent-stable protease was partially associated with the growth of Bacillus aquaemaris DS1.Based on the results of batch fermentation experiments,the Logistic equation and Luedeking-Piret equation were used to establish kinetic models for bacterial growth,organic solvent-stable protease production and substrate consumption.Good agreement was observed between the experimental and the predicted data of residual sugar concentration with a relative error of less than 10%.

关 键 词：有机溶剂稳定性脂肪酶 BACILLUS aquaemaris DS1 动力学模型 海洋细菌 

分 类 号：Q815[生物学—生物工程]