新型宫颈癌治疗性疫苗肿瘤杀伤作用  

Cytotoxic effect of a novel therapeutic vaccine on cervical cancer

作　　者：张卓梅[1] 宋海晶[2] 李娜[3] 邹佳[3] 季颖 王清清[3] 陈方[3] 高新[3] 董立厚[3] 

机构地区：[1]武警总医院妇产科,北京100039 [2]解放军第306医院急诊部,北京100106 [3]军事医学科学院放射与辐射医学研究所药理毒理研究室,北京100850 [4]上海泽润生物科技有限公司,上海201203

出　　处：《国际药学研究杂志》2012年第6期503-506,共4页Journal of International Pharmaceutical Research

基　　金：国家科技重大专项课题临床前药物代谢技术平台资助项目(2009ZX09304-004)

摘　　要：目的研究新型治疗性疫苗NTV的体内外宫颈癌杀伤作用。方法采用51Cr释放法研究脾淋巴细胞对宫颈癌细胞TC-1的体外杀伤活性,将正常小鼠体内激活的免疫活性细胞输给荷瘤小鼠,采用过继性细胞毒性T淋巴细胞(CTL)转移实验研究CTL的体内抗肿瘤作用。结果 CTL杀伤实验设置的效应细胞与靶细胞数量比分别为3:1、10:1及30:1,51Cr释放分析结果显示,疫苗NTV能够显著诱导脾淋巴细胞的肿瘤特异性杀伤作用,杀伤作用呈剂量依赖性变化,杀伤效率最高可达到53.5%;疫苗NTV在过继性CTL转移实验中的肿瘤抑制率为64.7%,上述结果均与阴性对照组有统计学差异(P<0.05)。结论疫苗NTV在体内外对宫颈癌细胞均具有良好的杀伤作用。Objective To evaluate cytotoxic effect of a novel therapeutic vaccine NTV on cervical cancer in vivo and in vitro. Methods 51 Cr release assay was used to study the cytotoxic activity of splenic lymphocytes targeted at TC-1 tumor cells.The method was used as a gold standard due to its good specificity and reproducibility.In addition,adoptive T cell transfer experiment was used to study the antitumor effect of cytotoxic T lymphocytes in vivo.Results In 51 Cr release assay,the ratio of effector cells to tumor cells（E∶ T） was 3∶ 1,10∶ 1 and 30∶ 1.The result showed that NTV could induce antitumor effect of splenocytes in vitro.Cytotoxic activity increased with the increase of E∶ T and dosage immunized with NTV,and the maximum cytotoxic activity was 53.5%.The result of adoptive T cell transfer experiment also showed splenocytes from mice immunized with NTV exhibited a good antitumor activity.The inhibition rate of NTV was 64.7% in adoptive T cell transfer experiment.The results mentioned above had a statistically significant difference compared with the control group（P 0.05）.Conclusion The results show that the therapeutic vaccine NTV exhibits a high cytotoxic effect on cervical cancer in vivo and in vitro,and good prospects for the treatment of cervical cancer.

关 键 词：治疗性疫苗 融合蛋白 抗肿瘤药 细胞毒作用 

分 类 号：R737.33[医药卫生—肿瘤]