1株拮抗酵母菌对柑橘绿霉病菌的抑制作用  

Inhibitive activity of Kluyveromyces marxianus on Penicillium digitatum Sacc.

作　　者：罗建军[1] 耿鹏[1] 胡美英[1] 黄慧斯[1] 贾建文[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《华中农业大学学报》2013年第1期54-58,共5页Journal of Huazhong Agricultural University

基　　金：广东省科技计划项目(2008B020900007)

摘　　要：以柑橘绿霉病菌(Penicillium digitatum Sacc.)为供试材料,研究拮抗酵母菌(Kluyveromyces ma-rxianus)对柑橘采后绿霉病菌的抑制效果,并观察其对采后柑橘果实多酚氧化酶(PPO)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性的影响。结果表明:拮抗酵母菌的菌株悬浮液和培养原液对柑橘采后绿霉病菌均有较好的抑制作用,但菌株培养上清液和热杀死液对柑橘绿霉病菌的抑制效果较差;与对照相比,接种拮抗菌与病原菌能够诱导柑橘果实PPO、POD和PAL活性的升高;拮抗酵母菌对柑橘绿霉病菌的抑菌机制与营养和空间的竞争以及诱导抗病性相关酶有关。The inhibitive activity of Kluyveromyces marxianus against citrus green mold caused by Penicillium digitatum Sacc. was investigated, as well as its effect on the activities of polyphenol oxi- dase （PPO）, peroxidase （POD） and phenylalanine ammonium-lyase （PAL）. The results showed that the treatments of washed cell suspension and living cell suspension have better inhibiting effect, as com- pared to the control treatment~ while the culture filtrate and autoclaved cell suspensions could exacer- bate green mold decay. The application of K. marxianus could induce higher PPO, POD and PAL activ- ity as compared to the control. It was suggested that the biocontrol mechanism might be competitive for nutrition and space, and contribute to the secretion of antagonistc substance.

关 键 词：柑橘绿霉病 马克斯克鲁维酵母 柑橘绿霉病菌 抗性酶 

分 类 号：S482.292[农业科学—农药学]