口灌氟苯尼考在黄鳝体内的药物代谢动力学及其残留  

Pharmacokinetics and elimination regularity of florfenicol and florfenicol amine,in rice field eel(Monopterus albus)

作　　者：谢玲玲[1] 吴志新[1] 袁娟[1] 刘佳佳[1] 彭小云[1] 朱旭[1] 陈孝煊[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2013年第1期97-102,共6页Journal of Huazhong Agricultural University

基　　金：国家科技支撑计划项目(2007BAD37B02);湖北省"十一五"科技攻关项目(2006AA203A02;2007AA203A01);华中农业大学研究生教育创新工程基金

摘　　要：采用高效液相色谱法,研究25℃下黄鳝单次口灌氟苯尼考(20mg/kg)后其体内的药物代谢和连续3d口灌(20mg/(kg.d))氟苯尼考后的药物残留消除规律。试验结果表明,氟苯尼考在黄鳝体内吸收迅速、组织分布广泛。血浆、肝脏、肾脏和肌肉中氟苯尼考的达峰质量浓度(Cmax)和达峰时间(Tmax)分别为6.07μg/mL、7.57、9.34、5.87μg/mg和3.67、4.45、2.01、9.28h。各个组织消除半衰期t1/2β)的大小顺序为肾脏(29.26h)>肝脏(19.32h)>肌肉(17.22h)>血浆(10.84h)。氟苯尼考胺在黄鳝体内代谢缓慢,肾脏中的消除半衰期和代谢率(MR)最高,分别为45.93h和49.21%。连续3次口灌后,标示残留物在黄鳝肾脏组织的残留量最高,其次为肝脏和肌肉。给药10d后所有组织均检测不到残留。若按照日本规定0.1mg/kg的最大残留限量(MRL)计算,得到的休药期为12d,与实测浓度判断法得出的休药期基本一致。The aim of this study was to evaluate the pharmacokinetics of florfenicol （FF） and its metabolite,florfenicol amine （FFA）,after oral （p. o. ） administration and investigate their depletion af- ter multiple oral administrations for 3 consecutive days in rice field eel （Monopterus albus） at 25 ℃. Af- ter p. o. administration of 20 mg/kg, the peak concentration of FF （Cmax） and time to reach FF peak con- centration （Tmax） in plasma, liver, kidney and muscle were 6.07 mg/L, 7.57 mg/kg, 9. 34 mg/kg, 5.87 9g/rag and 3. 67 h,4. 45 h, 2.01 h, 9. 28 h, respectively. The order of t1/2β was kidney （29.26 h）〉liver （19.32 h）〉muscle （17.22 h）〉plasma （10. 84 h）. FFA was also found in all tissues with a lower con- centration than FF after p. o. administrations throughout the study. The longest t1/2β of 45.93 h and the largest metabolic rate （MR） of 49. 21% were observed in kidney. After multiple oral doses （20 mg/（kg d） for 3 d） ,the highest residues was observed in kidney,next in liver and then in muscle. The residues was undetectable in all tissues after 10 d which was similar to the former 12 d withdrawal time to ensure that the residues of florfenicol are less than the maximal residue limit of tolerance established by Japan.

关 键 词：药物代谢动力学 黄鳝 氟苯尼考 氟苯尼考胺 残留 

分 类 号：S948[农业科学—水产养殖]