葛根芩连汤药效组分抑菌生物效价测定  

Determination of biological titers of active components alignments in Gegen Qinlian Decoction on bacteriostasis

作　　者：徐蓓蕾[1,2] 张贵君[1] 崔向微 文春先[1] 刘长松[1] 张雅楠[1] 

机构地区：[1]北京中医药大学,北京100102 [2]哈尔滨商业大学,哈尔滨150076 [3]北京建生药业有限公司,北京100039

出　　处：《中华中医药杂志》2013年第1期230-233,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省教育部产学研结合项目(No.2007B090400085)~~

摘　　要：目的:对葛根芩连汤药效组分抑菌的生物效价进行测定。方法:采用体外抑菌实验,观察葛根芩连汤及其药效组分对相关细菌的抑制作用,通过MIC和MBC值的测定及相应抑菌圈直径的测定,计算生物效价。结果:抑菌实验表明,葛根芩连汤及其药效组分对金黄色葡萄球菌、乙型链球菌、大肠埃希菌、痢疾杆菌等多种菌敏感,且药效组分抑菌效果明显优于复方水煎液,最终测得每1g药效组分相当于4.5倍葛根芩连汤(219g);每30mg药效组分相当于910U庆大霉素。结论:葛根芩连汤及其药效组分有较强的抗菌作用。Objective： To study the bacteriostatic effect of Gegen Qinlian Decoction and active components alignments（ACA） and determination of the biological titer of ACA.Methods： We observed the inhibition of bacteria by the extraneous bacteriostatic test,determined MIC and MBC and the diameter of bacteriostasis circle,and calculated biological titers.Results： Gegen Qinlian Decoction and ACA had sensitivity to staphylococcus aureus,beta streptococcus,escherichia coli,dysentery bacilli etc.,and the ACA was better than Gegen Qinlian Decoction.1g ACA was equal to 4.5 times Gegen Qinlian Decoction（219g）.30mg was equal to 910U gentamicin.Conclusion： Gegen Qinlian Decoction and ACA had stronger antibacterial action.

关 键 词：葛根芩连汤 药效组分 抑菌 生物效价 

分 类 号：R284[医药卫生—中药学]