丙二醛对小鼠骨髓间充质干细胞成骨分化的影响  

Effects of Malondialdehyde on the Osteogenic Differentiation of Mouse Bone Marrow-derived Mesenchymal Stem Cells

作　　者：李烨[1] 杨沐思[1] 刘文锋[1] 杨慧[1] 刘如石[1] 汪保和[1] 印大中[1,2] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,中国湖南长沙410081 [2]暨南大学附属清远市人民医院,中国广东清远511518

出　　处：《生命科学研究》2012年第6期496-500,共5页Life Science Research

基　　金：国家自然科学基金资助项目(31271257;31072141);湖南省自然科学基金资助项目(11JJ6020;12JJ2013;11JJ6082);2011年湖南省大学生研究性学习和创新性实验计划项目(77)

摘　　要：为了探索氧化应激活性中间产物丙二醛对小鼠骨髓间充质干细胞成骨分化的影响及机制,体外培养的间充质干细胞经丙二醛处理后,在第4、7、14、21 d分别提取等份细胞进行碱性磷酸酶活性检测,第7 d时提取RNA通过实时定量RT-PCR测定ALP和Runx2/Cbfal mRNA表达,并在第21 d进行von Kossa染色,茜素红染色.研究发现:丙二醛通过降低碱性磷酸酶活性及ALP和Runx2/Cbfal mRNA的表达,抑制矿化骨节形成.这些结果表明:丙二醛可通过抑制ALP和Runx2/Cbfal通路,抑制小鼠骨髓间充质干细胞的成骨分化.To explore the role and mechanism of malondialcehyde,a reactive intermediate of oxidative stress,in the osteogenic differentiation of murine bone marrow-derived mesenchymal stem cells（MSCs）,the in vitro cultured MSCs were incubated with malondialdehyde.Then,on the 4th,7th,14th and 21st day,the changes of alkaline phosphatase（ALP） activity were determined respectively.On the 7th day,mRNA was ex-tracted from the cells,ALP and Runx2/Cbfal mRNA expression were analyzed by Q-RT-PCR;and on the 21st day,the osteoblastic phenotype was verified by the von Kossa staining and the Alizarin Red S staining.The results showed that malondialdehyde could reduce the activity of alkaline phosphatase and the mRNA ex-pression of Runx2 and ALP,so as to inhibit the formation of mineralized nodules.The results indicated that the inhibiting mechanism of the osteogenic differentiation of murine MSCs by malondialdehyde may,at least in part,through suppressing key transcription factors of Runx2/Cbfal and ALP.

关 键 词：丙二醛 骨髓间充质干细胞 成骨分化 RUNX2 CBFAL 

分 类 号：Q955[生物学—动物学]