日本血吸虫可溶性虫卵抗原干预ApoE^(-/-)小鼠动脉粥样硬化机制研究  

Anti-atherogenic effect and its mechanisms of soluble egg antigen of Schistosomia japonicum in ApoE^(-/-)mice

作　　者：章辉[1] 邢芸[2] 孔辉[3] 戴洋[1] 何伟[1] 葛颂[4] 朱荫昌[1] 

机构地区：[1]江苏省寄生虫病防治研究所,无锡214064 [2]中国药科大学生物技术实验室 [3]南京医科大学附属江苏省人民医院呼吸科 [4]南京医科大学附属江苏省人民医院神经内科

出　　处：《中国血吸虫病防治杂志》2012年第6期654-658,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81001427;81000119)

摘　　要：目的探讨日本血吸虫可溶性虫卵抗原(SEA)对ApoE-/-小鼠动脉粥样硬化的保护作用及其免疫调节机制。方法设置实验组1和2,实验组1小鼠从第1周开始高脂饮食喂养,分为预防组和对照组,第1周时分别腹腔注射SEA及PBS,每周1次,共4次;实验组2小鼠高脂饮食喂养至第14周,分为治疗组和对照组,第14周时分别注射SEA及PBS,每周1次,共4次。第22周处死小鼠,观察SEA对动脉粥样硬化的干预作用以及小鼠体内的细胞因子和调节性T细胞亚群CD4+CD25+FoxP3+T细胞水平的变化。结果给予SEA后,与相应对照组比较,预防组和治疗组小鼠血清总胆固醇含量均显著降低,外周血TNF-α、IL-10水平均显著下降;预防组小鼠动脉粥样硬化斑块面积被显著抑制,但治疗组无明显变化。第22周时,预防组外周血CD4+CD25+FoxP3+T细胞占CD4+T细胞比例为(4.4±0.9)%,与对照组[(2.6±0.3)%]相比,差异有统计学意义(P<0.05);但治疗组细胞比例给予SEA前后差异无统计学意义(P<0.05)。结论SEA能降低ApoE-/-小鼠血清总胆固醇水平;预防性给予SEA具有抑制动脉粥样硬化作用,其主要机制是在疾病初始阶段抑制炎症因子产生Treg细胞增殖。Objective To study the preventive effects of soluble egg antigen（SEA） of Schistosomia japonicum on atherosclerosis in ApoE-/-mice and its immune modulatory mechanisms.Methods ApoE-/-mice were divided into an Experimental Group One and an Experimental Group Two.The mice in the Experimental Group One dividing into a prevention and a control subgroups were fed with high fat diet since the first week,the mice in the former subgroup were injected intraperitoneally with SEA while those in the latter one were injected with phosphate buffered saline（PBS） with 1 week interval for 4 times.The mice in the Experimental Group Two were fed with high fat diet for 14 weeks,and then they were divided into a treatment and a control subgroups,which were injected with SEA and PBS,respectively,since the 14th week with 1 week interval for 4 times.All the mice were killed in the 22nd week,and the atherosclerosis development and the change of levels of cytokines and CD4＋CD25＋ FoxP3＋T cells in mice were observed.Results Immunization with SEA led to a significant reduction in the levels of cholesterol,TNF-α,and IL-10 in all the ApoE-/-mice.The atherosclerosis plaque area of aorta of ApoE-/-mice in the prevention subgroup reduced obviously,while there was no significant change in the treatment subgroup.In the 22nd week,the proportion of CD4＋CD25＋ FoxP3＋T cells population in CD4＋ T cells was（4.4±0.9）% in the prevention subgroup and there was a significant difference compared with that of the control subgroup [（2.6±0.3）% ]（P 0.05）.However,the change of the proportion in the treatment subgroup showed no statistic significance（P 0.05）.Conclusion The preventive injection of SEA in ApoE-/-module mice has the effect of anti-atherosclerosis by increasing the proportion of Treg cells together with the inhibition of inflammatory cytokines at the beginning of disease.

关 键 词：日本血吸虫 可溶性虫卵抗原 动脉粥样硬化 免疫调节 细胞因子 调节性T细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]