吗啡条件性位置偏爱大鼠纹状体多巴胺递质和多巴胺D2受体表达的变化  

Changes of dopamine neurotransmitter and its receptor in striatum from conditioned place preference of rats administrated of morphine

作　　者：杨培润[1] 钱刚[1] 罗素元[1] 杨明理[1] 白威峰[1] 吴明松[1] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,遵义563099

出　　处：《中华行为医学与脑科学杂志》2012年第12期1067-1069,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金项目（30860373）

摘　　要：目的观察吗啡条件性位置偏爱（conditioned place preference，CPP）大鼠纹状体中多巴胺（DA）递质和多巴胺D2受体的同步变化，从神经递质和受体分子水平探讨通过纹状体多巴胺能系统的阿片类精神依赖机制。方法吗啡剂量递增注射10d，建立大鼠吗啡CPP模型，生理盐水对照组和吗啡模型组各取10只断头处死取脑纹状体，采用高效液相色谱法测定其多巴胺含量；另各取6只采用免疫组化检测纹状体中D2受体的表达。结果与生理盐水对照组比较[DA：（5．23±1．01）；D2受体：（0．08±0．02）]，模型组大鼠纹状体多巴胺递质含量增加（7．63±0．98）；多巴胺D2受体平均吸光度值下调（0．06±0．02），均差异有统计学意义（P〈0．01）。结论纹状体多巴胺递质含量上升和D2受体表达水平下调的同步变化在一定程度上参与了吗啡精神依赖的形成。Objective To observe the level of dopamine and its receptor in striatum from conditioned place preference （CPP） of rats administrated of morphine. And explore the mechanism of opioid-psychic dependence involving the levels of neurotransmitter and receptor. Methods CPP model was validated in morphine-dependence rats for 10 days. Striatum samples were harvested from two separate groups involving the saline group and morphine treated group （ n= 10 per experiment）. The DA contents in striatum were detected in rats with colorimetry. Immunohistochemistry was applied to detect the differential expression levels of dopamine receptor 2 （ DRD2 ） in samples. Results Compared with the saline group, higher content of neurotransmitter dopamine was observed in the morphine dependent rats （7.63 ±0.98 vs 5.23 ± 1.01, P〈0.01 ） ,while the expression level of DRD2 was down regulated （0.06 ± 0.02 vs 0.08 ± 0.02, P 〈 0.01 ）. Conclusion The increased expression of neurotransmitter dopamine and the decreased of DRD2 may be contributed to morphine-induced psychological dependence in morphine dependent rats.

关 键 词：吗啡 精神依赖 多巴胺 多巴胺D2受体 纹状体 

分 类 号：R749.6[医药卫生—神经病学与精神病学]