二核苷酸STR基因座D6S261的多态性检测  

Genetic Polymorphisms of the Dinucleotide STR Locus D6S261

作　　者：杨鹏[1,2] 刘亚楠[2] 聂燕钗[1,2] 周怀谷[2] 赵子琴[1] 

机构地区：[1]复旦大学上海医学院法医学系,上海200032 [2]上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海200083

出　　处：《法医学杂志》2012年第6期445-447,共3页Journal of Forensic Medicine

基　　金：上海市科学技术委员会科研计划项目(09231203600)

摘　　要：目的研究二核苷酸STR基因座在亲子鉴定中的应用。方法选取二核苷酸STR基因座D6S261,采取200份随机血样,家系样本16份,亲子鉴定193份血样进行扩增,采用3130XL遗传分析仪收集数据,PowerStats v12计算群体遗传学参数。结果获取15种等位基因及50种基因型,H为0.850、DP为0.953、PE为0.695、PIC为0.820。家系样本和亲子鉴定样本分型结果均符合遗传定律,未发现突变。结论 D6S261具有良好的遗传多态性,具有突变率低、稳定性好的特点,若进一步降低影子带(stutter bands)干扰,可作为解决亲权鉴定中基因突变难题的有效手段。Objective To investigate the application of dinucleotide STR locus in paternity testing. Methods Dinucleotide STR locus D6S261 was selected and the paternity testing blood samples were amplified using 200 random blood samples, 16 family samples and 193 paternity test samples. Data of the PCR products were collected by 3130XL Genetic Analyzer and the genetic parameters of population were calculated by PowerStats vl2. Results Fifteen alleles and 50 genotypes were found and H, DP, PE and PIC were 0.850, 0.953, 0.695, and 0.820, respectively. The typing results of both family samples and paternity test samples were accord with the law of inheritance, which no mutation was discovered. Conclusion The genetic polymorphisms of D6S261 show good characteristics with low mutation rate and high stability. It can be an effective method to solve the indetermination caused by mutation in paternity testing if the stutter bands can be decreased.

关 键 词：法医遗传学 多态现象 遗传 二核苷酸 亲子鉴定 

分 类 号：R394[医药卫生—医学遗传学]