新疆喀什地区维吾尔族18个STR基因座的遗传多态性  

Genetic Polymorphisms of 18 STR Loci in Uygur Population of Kashi Prefecture of Xinjiang

作　　者：张晓红[1,2] 李平 翁玮霞[1] 刘长晖[4] 刘宏[4] 唐建新[1,2] 刘超[4] 

机构地区：[1]南方医科大学法医学系,广东广州510515 [2]广州市公安局番禺分局,广东广州511400 [3]新疆喀什地区公安局,新疆喀什844000 [4]广州市刑事科学技术研究所,广东广州510030

出　　处：《法医学杂志》2012年第6期451-455,共5页Journal of Forensic Medicine

基　　金：广东省重点科技项目(2010A060801001);新疆维吾尔自治区科技支疆计划项目(201091143)

摘　　要：目的调查新疆喀什地区维吾尔族无关个体18个STR基因座（DJ8s51、D21Sll、D3S1358、FGA、D8S1179、vWA、CSFIPO、D16S539、D7S820、D13S317、D5S818、D2S1338、D19S433、D12S391、TPOX、TH01、PentaE和D6S1043）的遗传多态性并研究其法医学应用价值。方法采用DNATyperTM15Plus试剂盒对1381名维吾尔族无关个体血样进行复合扩增，3130XL遗传分析仪对扩增产物检测，GeneMapperIDv3．2软件进行基因分型。计算群体遗传学参数，并与其他人群进行比较，计算Reynold’s遗传距离，绘制系统发生树。结果在1381名维吾尔族无关个体中．共发现231种等位基因，单个等位基因频率分布在0．0004～0．5304，H在0．644～0．923，PIC在0．587～0．918，DP在0．817～0．988，CPE大于0．9999999。与广州汉族人群遗传距离最大（0．0883），与希腊人群遗传距离最小（0．0503）。结论这18个STR基因座在新疆喀什地区具有高度多态性，可满足该群体个体识别和亲权鉴定。其遗传多态性更接近欧洲。Objective To investigate the genetic polymorphisms of 18 STR loci （D18S51, D21Sll, D3S1358, FGA, D8Sl179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, D2S1338, D19S433, D12S391, TPOX, THO1, Penta E and D6SI043） in unrelated Uygur individuals in Kashi prefecture of Xinjiang and to explore the application value in forensic practice. Methods Blood samples from 1 381 unrelated Uygur individuals were amplified by using DNA TyperTM 15 Plus kit. The amplified products were detected by using 3130XL Genetic Analyzer and the genotyping was done by using GeneMapper ID v3.2. Population genetics parameters were calculated and compared with that of the other population. The genetic distance of Reynold＇s was calculated and phylogenetic tree was constructed at last. Results Of the 1 381 unrelated Uygur individuals, 231 alleles were detected, with an allele frequency of 0.0004-0.5304. The H values were 0.644-0.923, PIC values were 0.587-0.918, and DP values were 0.817-0.988, respectively, with a CPE〉0.9999999. The genetic distance was the longest （0.0883） to Guangzhou HaM population and the closest （0.0503） to Greek. Conclusion The 18 STR loci in the Uygur population of Kashi prefecture of Xinjiang have high genetic polymorphisms which are close to Europeans, and can be satisfied as genetic markers of population individual identification and paternity testing.

关 键 词：法医遗传学 短串联重复序列 多态现象 遗传 新疆[维吾尔自治区] 维吾尔族 

分 类 号：R394[医药卫生—医学遗传学]