离子液体-超声辅助萃取-高效液相色谱测定桑叶中黄酮  

Ionic Liquid-based Ultrasonic-Assisted Extraction Coupled with HPLC for Sensitive Determination of Flavonoids in Mulberry Leaves

作　　者：陈君[1] 周光明[1] 杨远高[1] 张丽贤[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715

出　　处：《食品科学》2012年第24期215-218,共4页Food Science

基　　金：国家科技重大专项(2008ZX07315)

摘　　要：目的:建立以离子液体1-丁基-3-甲基咪唑溴化盐([BMIM]Br)为萃取剂,结合超声辅助萃取,利用高效液相色谱法同时分离测定桑叶中的桑色素、槲皮素和山奈酚3种黄酮的方法。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸溶液,采用梯度洗脱;流速1.0mL/min;紫外检测波长365nm;柱温为34℃。结果:该方法对桑色素、槲皮素和山奈酚分别在0.0065～130、0.0064～128、0.0042～84μg/mL范围内线性关系良好,相关系数(r)均大于0.9998,检出限(RSN=3)依次为0.99、1.31、0.64ng/mL;样品回收率为90.00%～95.31%。结论:本法操作简单快速、定量准确、灵敏度高、成本低、且对环境友好,为桑叶中黄酮化合物的检测及分离提供了一个有效的科学方法。An analytical method to determine flavonoids such as morin hydrate, quercetin and kaempferol in mulberry leaves was proposed using ultrasonic-assisted extraction coupled with HPLC. The extraction solvent used was 1-butyl-3- methylimidazolium chloride （[BMIM]Br）. The chromatographic separation was performed on Phenomenex C18 column （250 mm×4.6 mm, 5 μm） using a mobile phase made up of methanol and 0.1% phosphoric acid at a flow rate of 1.0 mL/ rain by gradient elution. The detection wavelength and column temperature were set as 365 nm and 34 ℃, respectively. This method showed good linearity in the range of 0.0065-130μg/mL （r = 0.9999） for morin hydrate, of 0.0064-128 μg/mL （r = 0.9999） for quercetin, and of 0.0042-84 μg/mL for kaempferol. The limit of detection, LOD, （R_SN = 3） of this method was 0.99, 1.31 ng/mL and 0.64 ng/mL for morin hydrate, quercetin and kaempferol, respectively. Average recoveries ranging 90.00% to 95.31% were obtained for these three flavonoids （n = 3）. This method was simple, fast, accurate, sensitive, cheap and environment-friendly and could provide a scientific way to determine flavonoids in mulberry leaves.

关 键 词：离子液体 超声辅助 高效液相色谱 黄酮 桑叶 

分 类 号：O652.62[理学—分析化学]