Analysis of the Activation of the Nrf2-ARE Pathway Following Optic Nerve Injury in Mice  

作　　者：Feng Yan Suihua Chen Xiangjun Ji Chuang Nie Hainan Xie 

机构地区：[1]Department of Ophthalmology,Jinling Hospital [2]Department of Neurosurgery,Jinling Hospital,School of Medicine,Nanjing University

出　　处：《Eye Science》2012年第4期161-164,共4页眼科学报（英文版）

基　　金：Program grant by National Natural Science Foundation (No.81070974)

摘　　要：Purpose:The Nrf2-ARE pathway plays a cytoprotective role in many tissues,but its protective function in the optic nerve is unclear. The purpose of the study is to investigate the changes in activation of the Nrf2-ARE pathway following optic nerve injury (ONI) in mice. Methods:Using ONI mice models,the expression levels of Nrf2 in optic nerves were determined by real-time PCR at various time points. Results:The expression of Nrf2mRNA was significantly up-regulated at 1 d after ONI, peaking at 30 min after ONI. Conclusion:The Nrf2-ARE pathway was activated after ONI, providing evidence for the study of the protection and underlying mechanism of Nrf2-ARE pathway on optic nerves.Purpose： The Nff2-ARE pathway plays a cytoprotective role in many tissues, but its protective function in the optic nerve is unclear. The purpose of the study is to investigate the changes in activation of the Nrf2-ARE pathway following optic nerve injury （ONI） in mice. Methods： Using ONI mice models, the expression levels of Nrf2 in optic nerves were determined by real-time PCR at various time points. Results：The expression of Nrf2mRNA was significantly up- regulated at 1 d after ONI, peaking at 30 min after ONI. Conclusion： The Nrf2-ARE pathway was activated after ONI, providing evidence for the study of the protection and underlying mechanism of Nrf2-ARE pathway on optic nerves.

关 键 词：视神经损伤 小鼠模型 通路 激活 保护作用 实时PCR 保护功能 时间点 

分 类 号：R774.6[医药卫生—眼科]