结直肠癌患者血清miR-29a水平测定的临床意义  

The Clinical Significance of Serum miR-29a for Colorectal Cancer

作　　者：奉林 庞智[1] 沙莎[1] 吴兵[1] 徐峰[1] 尹少朋[1] 

机构地区：[1]苏州市立医院北区,苏州215000

出　　处：《航空航天医学杂志》2012年第12期1414-1416,共3页Journal of Aerospace medicine

摘　　要：目的:本研究的目的是探讨血清miR-29a在CRC诊断和预后判断中的价值。方法:选取无转移的结直肠癌患者50例和存在肝转移的患者48例,同时募集50名健康志愿者为对照组。应用实时荧光定量PCR法检测miR-29a水平。结果:结直肠癌患者的血清miR-29a水平显著高于健康对照者(P<0.05),进行结直肠癌诊断时的灵敏度为71.35%,特异度为83.96%。结直肠癌肝转移的患者血清miR-29a水平显著高于未转移的CRC患者(P<0.01)。鉴别转移性与非转移性CRC患者的敏感性为79.38%,特异性为85.26%。结论:血清miR-29a可能是一种新的非侵入性指标用于结直肠癌患者的早期诊断,血清中的miR-29a水平升高与结直肠癌患者预后有关。Objective： The purpose of this study was to investigate the value of diagnosis and prognosis prediction of ser- um miR -29a for colorectal cancer. Methods：Serum miR -29a was detected by using real - time RT - PCR, corre- sponding 98 CRC patients, including 48 CRC patients with liver metastasis and 50 healthy volunteers. Results： Serum miR -29a was significantly higher in CRC patients than in controls（ P 〈 0.05 ）. The sensitivity was 71.35% and the spe- cificity was 83.96% in discriminating CRC patients from health controls. In addition, increased levels of miR - 29a ex- pression were also observed in colorectal tumors from colorectal liver metastasis patients compared with CRC patients （P 〈 0.01 ）. The sensitivity was 79. 38%＇ and the specificity was 85.26% in discriminating metastatic from non, metastatic patients. Conclnsions：These findings suggest that serum miR- 29a has strong potential as a novel noninvasive biomarker for early diagnosis of CRC patients and high levels of serum miR - 29a were associated with poor prognosis.

关 键 词：结直肠癌 血清 微小RNA 生物学标记 诊断 

分 类 号：R735.3[医药卫生—肿瘤]