CRE和AP-1元件参与人hsp90β基因的表达  

CRE and AP-1 elements in the expression of human hsp90β gene

作　　者：刘秉胜[1] 吴宁华[1] 沈珝琲[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2000年第3期28-31,共4页Basic and Clinical Medicine

基　　金：自然科学基金!39770169;39930050;863课题!102-11-02-05

摘　　要：为了进一步阐明人热休克蛋白90β（hsp90β）基因的表达调控机制，本文对hsp90β基因调控序列中的CRE和AP－1元件进行了研究。构建了含有这两个元件的系列删切报告基因质粒，通过转染Jurkat细胞，检测并分析报告基因的活性。结果表明，CRE和AP-1元件均不同程度地参与了hsp90β基因的组成性、PHA激活和热诱导表达。Several reporter plasmids with deleting CRE and AP-1 elements in the regulation sequences of hsp90βgenewere constructed and transfected into Jurkat cells. Then the reporter genes' activity was datected. The results showthat CRE and AP-1 elements were critical to the high constitutive expression of hsp90β gene; in the PHA activatedexpression, CRE or AP-1 elememe play synergistic role; CRE and AP-1 elements also partially mediate in the heat shockinducible expression of hsp90β gene.

关 键 词：CRE元件 AP-1元件 HSP90Β基因 基因表达 

分 类 号：R363[医药卫生—病理学]