人巨细胞病毒感染与宫颈癌关系的研究  

Study on the Relationship between Human Cytomegalovirus Infection and Cervical Cancer

作　　者：闵洁[1] 林美华[1] 

机构地区：[1]同济医科大学附属协和医院妇产科,武汉430022

出　　处：《同济医科大学学报》2000年第3期249-252,共4页Acta Universitatis Medicinae Tongji

基　　金：湖北省自然科学基金资助项目!(No.99J150)

摘　　要：采用聚合酶链反应 (PCR)检测 30例宫颈癌、 30例宫颈炎、 8例正常宫颈组织标本中 HCMV早期 (IE)、晚期 (L)基因片段、 HCMV79- aa ORF癌基因及 HPV16、 18、 32、 5 2 b、 5 8型 DNA。结果表明 :除 HCMV L 基因外 ,以上各基因在宫颈癌组之检出率均显著高于宫颈炎组 ,且宫颈癌组 IE和 L 基因、HPV高危型和 HCMV同时感染的比值比 (OR)分别高于 IE或 L 基因、HPV高危型或 HCMV单独感染的 OR值之和。提示人巨细胞病毒与宫颈癌的发生有关 ,可通过其 IE基因启动、激活 HPV的致癌作用 ,HCMV79- aaPolymerase chain reaction (PCR) technique was used to detect human cytomegalovirus (HCMV) immediate early (IE) gene, late(L) gene, HCMV 79 aa ORF oncogene and human papillomavirus (HPV)16, 18, 32, 35, 52b, 58DNA in cervical specimens from 30 cases of cervical cancer, 30 cases of cervicitis and 8 cases of normal cervixes. The results showed that the positive rate for all the genes in cervical cancer specimens, except for HCMV L gene, was obviously higher than that in cervicitis ones. In cervical cancer specimens the mutual estimate odds ratios (OR) of HCMV IE and L genes was higher than the sum of their respective ORs in cervicitis specimens, so was the OR of high risk HPV types and HCMV co infecting. It was suggested that HCMV was etiologically associated with cervical cancer, wich was contributed to the tumorigenesis of initiation of IE gene and activation of HPV. The integration of HCMV 79 aa ORF oncogene was one of the ineglectable factors in oncogenesis.

关 键 词：巨细胞病毒 乳头瘤病毒 聚合酶链反应 宫颈癌 

分 类 号：R737.330.2[医药卫生—肿瘤]