MCAO大鼠梗死灶对侧皮层Nogo-A的动态变化及电针干预  

The active change of Nogo-A and acupuncture's efficacy in the contralateral cortex of rats after focal brain ischemia

作　　者：梁艳桂[1] 谭峰[1] 陈杰[1] 吴海科[1] 万赛英[1] 王海侨[1] 王学文[1] 黄勋福[1] 韩福兰[1] 吴强[2] 

机构地区：[1]广州中医药大学附属佛山市中医院神经内科,广东佛山528000 [2]中山大学电镜室,广东广州510080

出　　处：《中风与神经疾病杂志》2012年第12期1073-1076,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.8152800007000001);广东省自然科学基金资助项目(No.81072947)

摘　　要：目的观察脑缺血再灌注大鼠梗死灶对侧皮层不同时期轴突生长抑制因子Nogo-A的动态变化,并探讨电针对梗死灶对侧皮层Nogo-A的调节作用。方法采用改良Zea Longa法制备左侧大脑中动脉闭塞再灌注模型。采用免疫组织化学染色法及透射电镜技术观察电针对IR后1、7、28d各组大鼠脑梗死对侧皮层轴突再生抑制因子Nogo-A表达及神经元细胞形态、超微结构的影响。结果 (1)电针、假穴位、模型3组大鼠与假手术组大鼠相比,在IR后1d、7d梗死对侧皮层Nogo-A的表达均升高(P<0.05),7d最明显,第28天下降至基础水平(P>0.05);电针组大鼠IR后1d、7d脑梗死对侧皮层Nogo-A的表达均低于同期模型组和假穴位组,差异有统计学意义(P<0.05),但假穴位组与模型组同期对比差异无统计学意义(P>0.05)。(2)电针、假穴位、模型3组各时间点大鼠脑梗死对侧皮层出现神经元细胞胞体肿胀、细胞器水肿、核染色质轻度溶解、毛细血管内皮细胞肿胀、管腔狭窄等轻度病理改变,以缺血再灌注后第7天最明显,28d明显减轻。上述病理改变均较同期脑缺血区病变程度明显减轻,且不足以引起支配侧肢体神经功能缺失。电针组大鼠脑梗死对侧皮层神经元细胞、超微结构的改变均较同期模型组、假穴位组减轻。结论电针治疗脑梗死的有效机制可能与其减轻脑细胞超微结构的损害、下调中枢神经轴突生长抑制因子Nogo-A的表达、诱导神经轴突再生、减轻脑梗死后的远隔损害等密切相关。Objective To observe the influence of electro-acupuncture on expression of Nogo-A in the contralateral cortex at different time points after the cerebral ischemia-reperfusion in rats.Method The rats were adopted modified method of Zea Longa to prepare the left middle cerebral artery occlusion（MCAO）.Using the immunohistochemistry and the transmission electron microscopy to observe effect of electro-acupuncture after cerebral ischemia for 2h and reperfusion for 1d,7d and 28d.The expression of contralateral cortex Nogo-A of each group and effect of ultrastructure onset were compared with the model group and the false electroacupuncture group.Results On 1d and 7d after MCAO,compared with the sham operation group,the expression of Nogo-A positive cells in electro-acupuncture group,false electroacupuncture group and model group were increased（P0.05）.They increased significantly on 7d,and then decreased on 28d.On 1d,7d and 28 after MCAO,compared with the model group and false electroacupuncture group at the same time,the expression of Nogo-A positive cells in electroacupuncture group was lower,and there was statistical difference（P0.05）.Among electroacupuncture group,model group and false electro-acupuncture group,it appeared different levels of pathological changes such as the edema of ischemic areas in brain cortex,mild chromatolysis,edema of the endothelial cells of capillaries and the stenosis of vessel lumen at different time points.The changes were the most serious on 7d and significantly decreased on 28d after MCAO.The aboving pathological changes were all easer than that in the affected side at the same time,and did not cause the the dysfunction of nervous system.Compared with model group and false electro-acupuncture group,the injury of neurons,oligodendrocytes,capillaries and other ultramorphology in cerebral ischemia area were relatively easer than that in electroacupuncture group.Conclusion The mechanism that electro-acupuncture treating cerebral infarction probably be correlated with reducing th

关 键 词：脑缺血再灌注 轴突生长抑制因子Nogo-A 远隔损害 神经元 超微结构 电针 

分 类 号：R743.3[医药卫生—神经病学与精神病学]