17β-雌二醇对人根尖牙乳头干细胞RANKL、OPG表达影响的研究  

Effect of 17β-estrodial on the expression of RANKL and OPG in human stem cells from apical papillae

作　　者：王燕萍[1] 曹灵[1,2] 吴锦涛[1,3] 闫明[1] 景双林[1,3] 张光东[1,3] 于金华[1,3] 

机构地区：[1]南京医科大学口腔医学研究所,江苏南京210029 [2]南京医科大学附属口腔医院儿童口腔科,江苏南京210029 [3]南京医科大学附属口腔医院牙体牙髓病科,江苏南京210029

出　　处：《口腔生物医学》2012年第4期185-188,共4页Oral Biomedicine

基　　金：江苏省卫生厅医学重点人才项目(RC2011140);江苏高校优势学科建设工程资助项目

摘　　要：目的:探讨17β-雌二醇对人根尖牙乳头干细胞(stem cells from apical papilla,SCAPs)核因子-κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)、骨保护素(osteoprotegerin,OPG)表达的影响。方法:通过酶消化法分离培养SCAPs,将SCAPs分别于对照组(普通培养基+10μmol/L无水乙醇)和含0.1μmol/L17β-雌二醇的培养基培养3d和7d后,实时荧光定量PCR法和蛋白质免疫印迹法分别检测RANKL、OPG mRNA和蛋白表达。结果:17β-雌二醇刺激3d和7d组,OPGmRNA和蛋白表达均较对照组升高(P<0.01),RANKL mRNA和蛋白表达均较对照组降低(P<0.01)。结论:17β-雌二醇可能通过调节RANKL/OPG系统,参与调节SCAPs成牙/骨及破牙/骨活性。Objective：To evaluate the effects of 17β-estrodial on the expression of receptor activator of NF-κB ligand （RANKL） and osteoprotegerin （OPG） in human stem cells from apical papillae （SCAPs）. Methods：SCAPs were cultured seperately by enzyme digestion. SCAPs cultured in L-MEM containing 10 μmol/L alcohol （control group） , L-MEM containing 0.1μmoL/L 17β-estrodial for 3 days and 7 days were respectively collected to evaluate the expression of RANKL and OPG at mRNA and protein levels by real-time RT-PCR and Western blotting. Results：For SCAPs cultured in 17 β-estrodial for 3 days and 7 days, expression of OPG at mRNA and protein levels was all higher than those in control group （P 〈 0.01 ）,while expression of RANKL at mRNA and protein levels was all lower than those in control group （P 〈 0. 01 ）. Conclusions：17β-estrodial may regulate the odonto/osteogenic and odonto/osteoclastic potential of SCAPs through RANKL/OPG system.

关 键 词：17Β-雌二醇 人根尖牙乳头干细胞 核因子-ΚB受体活化因子配体 骨保护素 

分 类 号：R780.2[医药卫生—口腔医学]