肿节风复方对肝癌细胞HepG2作用效果观察  

Observation on the Effects of Sarcandrae Compound on HepG2 Cells

作　　者：朱晓莹[1] 龙昶文[2] 梁永赞[2] 彭彦桦[2] 李韬[1] 农嵩[1] 李朝敢[1] 张树球[1] 

机构地区：[1]右江民族医学院生化教研室,百色市533000 [2]右江民族医学院药学系,百色市533000

出　　处：《广西医学》2012年第12期1597-1599,共3页Guangxi Medical Journal

基　　金：右江民族医学院科研项目(科字[2011]1号)

摘　　要：目的探讨肿节风复方对人肝癌细胞HepG2细胞增殖及端粒酶活性的影响。方法以不同浓度肿节风复方处理肝癌HepG2细胞48 h,观察细胞形态学改变;采用MTT法评价不同浓度肿节风复方对HepG2细胞增殖的影响并计算半抑制浓度(IC50);应用ELISA法检测肿节风复方、紫杉醇对HepG2细胞端粒酶活性的影响。结果肿节风复方处理HepG2细胞48 h后,细胞脱落明显,增殖活力显著降低(P<0.05),呈现剂量依赖趋势;肿节风复方对HepG2细胞IC50为292.2 mg/ml。肿节风复方和紫杉醇对HepG2细胞端粒酶活性均有抑制作用(P<0.05),并且抑制作用相当(P>0.05)。结论肿节风复方能抑制HepG2细胞增殖及端粒酶活性。Objective To observe the effects of Sarcandrae Compound on proliferation and telomerase activity in HepG2 cells. Methods Human liver cancer HepG2 cells were treated with different concentrations of Sarcandrae Compound for 48 hours. Morphologic changes of cells were observed. MTY assay was used to evaluate the effects of different concentrations of Compound Sarcandrae on the proliferation of HepG2 cells and calculate ICs0. ELISA was applied to detect the telomerase activity of HepG2 cells inhibited by Sarcandrae Compound and Paclitaxel. Results After HepG2 cells were treated with Sarcandrae Compound for 48 hours, cells fell off obviously and proliferation activity reduced significantly（P 〈 0.05 ）, which presented a dose-dependent trend. ICs0 was 292.2 mg/ml. Sarcandrae Compound could significantly decrease the telomerase activity of HepG2 cells as well as Paclitaxel（P 〈 0.05 ）, and their inhibitory effects were similar（P 〉 0.05）. Conclusion Sarcandrae Compound can significantly inhibit the proliferation of HepG2 cells and their telomerase activity.

关 键 词：肿节风复方 肝癌细胞HEPG2 增殖 端粒酶 

分 类 号：R735.7[医药卫生—肿瘤]