芍药苷对过氧化氢诱导SH-SY5Y细胞PCNA及Caspase-3表达的影响  

Effect of Paeoniflorin on Expression of PCNA and Caspase-3 in Peroxide-induced SH-SY5Y Cells

作　　者：周洁[1] 曹鹏涛[1] 冯凯祥[1] 贾岩岩[1] 罗雪婷[1] 潘梦音[1] 温新丽[1] 于甜甜[1] 杨五彪[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2012年第4期241-243,共3页Journal of Henan University of Science & Technology:Medical Science

基　　金：国家自然科学基金项目(31171256);河南省医学科技攻关项目(201003079);河南科技大学大学生研究训练计划项目(2012192)

摘　　要：目的探讨芍药苷对过氧化氢(H2O2)诱导SH-SY5Y氧化损伤的保护作用及其可能机制。方法MTT法测定细胞存活率,细胞免疫化学技术检测细胞细胞核增殖抗原(PCNA)、Caspase-3的表达。结果 0.87mmol/L H2O2可使细胞存活率降低,PCNA的表达减少,Caspase-3的表达增加。经过39μg/mL和62.5μg/mL浓度的芍药苷处理后,H2O2诱导的SH-SY5Y细胞存活率明显升高,PCNA的表达明显增加,同时抑制Caspase-3的表达。结论芍药苷可抑制过氧化氢诱导的SH-SY5Y细胞凋亡,其作用机制可能与其增加细胞PCNA的表达,减少Caspase-3的表达有关。Objective To study the protective effect of paeoniflorin （PF） on hydrogen peroxide （I-I202 ）- induced oxidative injury in SH-SY5Y. Methods To measure the cell viability of SH-SY5Y with MTT assay, the expression of PCNA and Caspase-3 was determined with immunohistochemical method. Results 0. 87 mmol/L H2O2 could significantly induce oxidative injury of SH-SY5Y cell viability, decrease the expression of PCNA, increase the expression of Casepase-3. In the presence of 39,62.5μg/mL PF,H2O2- induced cell viability was increased. With immunocytochemical analysis, a up-regulation PCNA expression and a down-regulated Caspase-3 expression was observed. Conclusion PF could protect against H2O2 - induced SH-SYSY cell apoptosis, which mechanism is correlated with PCNA up-regulation and Caspase-3 down -regulation.

关 键 词：芍药苷 过氧化氢 SH-SY5Y细胞 凋亡 

分 类 号：R774[医药卫生—眼科]