毛细管气相色谱法在血液DHA、EPA测定中的应用及评价  

Application and evaluation of capillary gas chromatography for the determination of DHA and EPA in blood

作　　者：韩光辉 张艳丽 贾秋桦 公新 刘泽伦[2] 

机构地区：[1]临沂市妇幼保健院,山东临沂276000 [2]北京大学人民医院

出　　处：《山东医药》2012年第47期29-30,共2页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划项目(W1201325)

摘　　要：目的评价毛细管气相色谱法在血液二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)检测中的应用效果。方法建立毛细管气相色谱法,测定血液中的DHA、EPA,并对该方法进行评价。结果高中低三个浓度的加标回收试验,DHA平均回收率为92.0%～93.6%,RSD为3.0%～3.6%,EPA的平均回收率为92.3%～94.0%,RSD为2.8%～4.0%。结论毛细管气相色谱法精密度、准确度和重复性较好,且短时间内稳定,适用于检测血液中的DHA和EPA。Objective To evaluate the application effect of the determination of docosahexaenoic acid（DHA）and eicosapentaenoic acid（EPA） in blood by capillary gas chromatographic（CGC） method.Methods CGC method was established,and used to determinate the concentration of DHA and EPA in blood,then this method was evaluated.Results In the standard recovery test of high,middle and low levels,the DHA average recovery rate was between 92.0 % and 93.6%,RSD ranged from 3.0% to 3.6%.The EPA average recovery rate was between 92.3% and 94.0%,RSD ranged from 2.8% to 4.0%.Conclusion CGC method is precise,accurate,reproducible,and stable in a short time.It is suitable for the determination of DHA and EPA in blood.

关 键 词：毛细管气相色谱法 孕妇 二十二碳六烯酸 二十碳五烯酸 

分 类 号：R466.11[医药卫生—临床医学]